Normalization by cell fusion of sister chromatid exchange in Bloom syndrome lymphocytes.
Normalization by cell fusion of sister chromatid exchange in Bloom syndrome lymphocytes.
复制标题
布卢姆综合征淋巴细胞中姐妹染色单体交换的细胞融合正常化。
DOI：
10.1126/science.7221565
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Sandberg,AA
中科院分区：
文献类型：
--
作者：
Shiraishi,Y;Matsui,S;Sandberg,AA
Bloom综合征（BS）患者与正常人或BS杂合子的新鲜淋巴细胞融合导致BS细胞染色体中姐妹染色单体交换频率完全正常化。这种正常化发生在杂交细胞的第一次有丝分裂。与此相反，BS淋巴细胞与正常受试者或BS杂合子培养对姐妹染色单体交换没有影响。细胞融合实验表明，BS细胞中姐妹染色单体交换频率的正常化可以通过各种哺乳动物细胞中保守的因子来实现。这些发现与BS是在DNA修复功能的调节水平上的隐性遗传突变的概念是一致的。
Fusion of fresh lymphocytes from a Bloom syndrome (BS) patient with those of normal subjects or a BS heterozygote resulted in complete normalization of the frequency of sister chromatid exchanges in the chromosomes of BS cells. This normalization took place by the first mitosis in hybrid cells. In contrast, cultivation of BS lymphocytes with those of normal subjects or the BS heterozygote had no effect on sister chromatid exchanges. The cell fusion experiments suggest that the normalization of the sister chromatid exchange frequencies in BS cells can be achieved by factors conserved in the cells of various mammalian species. These findings are compatible with the concept that BS is a recessive genetic mutation at regulatory levels of the DNA repair function.