Elevated Levels of T-helper 17-associated Cytokines in Diabetes Type I Patients: Indicators for Following the Course of Disease
Elevated Levels of T-helper 17-associated Cytokines in Diabetes Type I Patients: Indicators for Following the Course of Disease
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DOI：
10.1080/08820139.2016.1197243
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发表时间：
2016-10-01
影响因子：
2.8
通讯作者：
Khoubyari, Mahshid
中科院分区：
文献类型：
--
作者：
Baharlou, Rasoul;Ahmadi-Vasmehjani, Abbas;Khoubyari, Mahshid
背景资料：1型糖尿病（T1 D）被认为涉及慢性炎症，其表现为不同炎症介质的激活和表达。Th 1和Th 17相关的细胞因子是已被证明发挥深刻的促炎活性的因子，并与小鼠和人类T1 D的发病机制有关。目的：因此，本病例对照研究的目的是测定血清IL-17、IL-21、IL-27、转化生长因子β（TGF-β）患者和方法：血液样本收集自48例T1 D患者和49名健康个体，基于简单抽样，没有恶性肿瘤或自身免疫性疾病的历史。采用酶联免疫吸附法（ELISA）检测血清IL-17、IL-21、IL-27、TGF-β、IFN-γ水平。与健康人相比，T1 D患者血清IL-17和IL-21水平显著升高（分别为p = 0.005和0.01），但有趣的是，IL-27的情况相反（p < 0.0001）。而TGF-β和IFN-γ在两组间无显著性差异。此外，IL-17/IFN-γ和IL-17/IL-27的比例较高的患者相比，对照group.Conclusions：我们的研究结果表明占主导地位的Th 17相关的IL-17，这表明从Treg和Th 1表型向Th 17表型的转变。因此，它可以促进T1 D细胞的炎症。此外，这表明Th 17和Th 17/Th 1比率作为细胞破坏的潜在贡献者的作用，并且Th 17/Th 1应答比率可以提供用于在细胞破坏和疾病进展到临床终末期之前快速诊断T1 D的新生物标志物。
Background: Type 1 diabetes (T1D) is thought to involve chronic inflammation, which is manifested by the activation and expression of different inflammatory mediators. Th1- and Th17-associated cytokines are factors that have been shown to exert profound pro-inflammatory activities and have been implicated in the pathogenesis of T1D in mice and humans.Objectives: Therefore, the aim of this case control study was to determine the serum level of IL-17, IL-21, IL-27, transforming growth factor beta (TGF-), and IFN- and their reciprocal relationship in Iranian T1D patients.Patients and Methods: Blood samples were collected from 48 T1D patients and 49 healthy individuals with no history of malignancies or autoimmune disorders based on simple sampling. The serum levels of IL-17, IL-21, IL-27, TGF-, and IFN- were measured by the enzyme linked immunosorbent assay (ELISA).Results: The serum levels of IL-17 and IL-21 were significantly higher in T1D patients compared to the healthy individuals (p = 0.005 and 0.01, respectively), but interestingly, the opposite was the case for IL-27 (p < 0.0001). However, there were no significant differences in TGF- and IFN- between both groups. In addition, IL-17/IFN- and IL-17/IL-27 ratios were higher in patients compared to the control group.Conclusions: Our results indicated dominant Th17-associated IL-17, suggesting a shift from the Treg and Th1 phenotypes toward the Th17 phenotype. Therefore, it can promote inflammation in cells in T1D. In addition, it suggests the role of Th17 and Th17/Th1 ratios as a potential contributor to cells destruction and the Th17/Th1 response ratio may provide a novel biomarker for rapid T1D diagnosis before the destruction of cells and progression of the disease to the clinical end stages.