Hipposin, a histone-derived antimicrobial peptide in Atlantic halibut (Hippoglossus hippoglossus L.)
Hipposin, a histone-derived antimicrobial peptide in Atlantic halibut (Hippoglossus hippoglossus L.)
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DOI：
10.1016/s1570-9639(03)00018-9
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发表时间：
2003-03-21
影响因子：
3.2
通讯作者：
Nissen-Meyer, J
中科院分区：
文献类型：
--
作者：
Birkemo, GA;Lüders, T;Nissen-Meyer, J
从大比目鱼（Hippoglossus acrylossus L.）采用酸提取、阳离子交换和反相色谱分离。AMP的完整氨基酸序列（称为马蛋白酶）通过自动Edman降解和质谱法测定为SGRGKTGGKARAKAKTRSSRAGLQFPVG-RVHRLLRKGNYAHRVGAGAPVYL。N-末端氨基被乙酰化。计算得到的海马苷的理论质量为5458.4 Da，与基质辅助激光解吸电离质谱（MALDI-MS）测定的质量5459 Da一致。通过PCR扩增大西洋比目鱼基因组DNA的编码序列，证明海马蛋白来源于组蛋白H2 A。该肽与亚洲蟾蜍的39-mer AMP buforin I和鲶鱼的19-mer AMP parasin I具有序列相似性。51个残基中的50个与虹鳟鱼组蛋白H2 A的N-末端区域相同。海马蛋白对几种革兰氏阳性和革兰氏阴性细菌显示出强的抗微生物活性，并且可以检测到低至0.3 μ M（1.6 μ g/ml）的海马蛋白浓度的活性。通过固相肽合成法制备了N-末端无乙酰化的海马蛋白，并显示其具有与天然乙酰化肽相同的抗菌活性。因此，海马蛋白是一种新的广谱组蛋白衍生的AMP中发现的皮肤粘液的大西洋大比目鱼。(C)2003 Elsevier Science B. V.保留所有权利。
A novel 51-residue antimicrobial peptide (AMP) from the skin mucus of Atlantic halibut (Hippoglossus hippoglossus L.) was isolated using acid extraction, and cationic exchange and reversed phase chromatography. The complete amino acid sequence of the AMP, termed hipposin, was determined by automated Edman degradation and mass spectrometry to be SGRGKTGGKARAKAKTRSSRAGLQFPVG-RVHRLLRKGNYAHRVGAGAPVYL. The N-terminal amino group was acetylated. The theoretical mass of hipposin was calculated to be 5458.4 Da, which was in good agreement with the mass of 5459 Da determined by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Hipposin was shown to be derived from histone H2A by PCR amplifying the encoding sequences from Atlantic halibut genomic DNA. The peptide showed sequence similarity with the 39-mer AMP buforin I of Asian toad and the 19-mer AMP parasin I of catfish. Fifty of the fifty-one residues in hipposin were identical to the N-terminal region of histone H2A from rainbow trout. Hipposin showed strong antimicrobial activity against several Gram-positive and Gram-negative bacteria and activity could be detected down to hipposin concentrations of 0.3 muM (1.6 mug/ml). Hipposin without N-terminal acetylation was prepared by solid-phase peptide synthesis and shown to have the same antimicrobial activity as the natural acetylated peptide. Thus, hipposin is a new broad-spectrum histone-derived AMP found in the skin mucus of Atlantic halibut. (C) 2003 Elsevier Science B.V. All rights reserved.