DIFFERENTIAL EXPRESSION OF INSULIN-LIKE GROWTH-FACTOR GENES IN RAT CENTRAL NERVOUS-SYSTEM
DIFFERENTIAL EXPRESSION OF INSULIN-LIKE GROWTH-FACTOR GENES IN RAT CENTRAL NERVOUS-SYSTEM
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DOI：
10.1073/pnas.85.1.265
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发表时间：
1988-01-01
影响因子：
11.1
通讯作者：
KRAUSE, JE
中科院分区：
文献类型：
--
作者：
ROTWEIN, P;BURGESS, SK;KRAUSE, JE
采用灵敏的溶液杂交技术研究了胰岛素样生长因子I和II（IGF-I和IGF-II）基因在大鼠中枢神经系统（CNS）中的表达。两种IGFs的mRNA在成年大鼠的整个CNS中合成，但每种生长因子表现出明显的区域差异。IGF-I mRNA在颈胸脊髓和嗅球中的丰度是全脑的8-10倍，在中脑和小脑中富集3倍。IGF-II mRNA在延髓-脑桥和小脑中最低限度地富集，但在中脑和纹状体中的丰度比在全脑中低3-5倍。在CNS发育过程中，IGF-I和IGF-II mRNA的含量在胚胎第14天最高，出生时下降3-4倍，达到成人大脑中的值。胚胎神经元和神经胶质细胞在短期细胞培养过程中合成IGF-I mRNA;只有神经胶质细胞产生IGF-II mRNA。这些观察结果表明，IGF-I和IGF-II在发育和成人CNS中差异表达，并表明每种生长因子可能在哺乳动物神经系统中发挥独特的作用。
A sensitive solution-hybridization assay was used to investigate the expression of genes encoding insulin-like growth factors I and II (IGF-I and -II) in the rat central nervous system (CNS). mRNAs for both IGFs are synthesized throughout the CNS of adult rats but exhibit distinct regional differences for each growth factor. IGF-I mRNA is 8-10 times more abundant in the cervical-thoracic spinal cord and in the olfactory bulb than in whole brain and is enriched 3-fold in the midbrain and cerebellum. IGF-II mRNA is minimally enriched in the medulla-pons and cerebellum but is 3-5 times less abundant in the midbrain and corpus striatum than in total brain. During CNS development the content of IGF-I and IGF-II mRNAs is highest at embryonic day 14 and declines by a factor of 3-4 at birth, to values found in adult brain. Embryonic neurons and glia synthesize IGF-I mRNA during short-term cell culture; only glia produce IGF-II mRNA. These observations show that IGF-I and IGF-II are differentially expressed in the developing and adult CNS and suggest that each growth factor may play a unique role in the mammalian nervous system.