A hand-held, real-time, AI-assisted capillary convection PCR system for point-of-care diagnosis of African swine fever virus
A hand-held, real-time, AI-assisted capillary convection PCR system for point-of-care diagnosis of African swine fever virus
复制标题
用于非洲猪瘟病毒即时诊断的手持式实时人工智能辅助毛细管对流 PCR 系统
DOI：
10.1016/j.snb.2022.131476
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发表时间：
2022-01-29
影响因子：
8.4
通讯作者：
Qiu, Xianbo
中科院分区：
文献类型：
--
作者：
Miao, Guijun;Jiang, Xiaodan;Qiu, Xianbo
开发了一种手持式、低成本、实时对流聚合酶链反应（CPCR）系统，用于非洲猪瘟（ASF）病毒的床旁诊断。在CPCR扩增中，由于热对流，试剂被反复转移到反应的变性、退火和延伸阶段。比较了两种不同的光纤光学检测模式，结果表明，与侧壁收集相比，底部收集可显著提高检测灵敏度、重复性和一致性。每个毛细管都由其自身的LED通过共同的滤光片从顶部照亮，同时，采用配备有另一个滤光片的相机来检测来自光纤的荧光信号。为了处理由于非特异性扩增导致的阴性检测的不典型实时荧光曲线，采用基于人工智能的分类方法，例如人工神经网络（ANN），以将检测特异性提高至97.50%，而基于Ct的方法达到47.5%或75.0%。采用智能手机来运行AI算法和定制软件，以及报告具有地理信息的ASF事件。实验结果表明，该系统能够在30 min内成功检测出ASF病毒，且具有良好的灵敏度和特异性。
A hand-held, low-cost and real-time convection polymerase chain reaction (CPCR) system is developed for point of-care diagnosis of African swine fever (ASF) virus. In CPCR amplification, the reagent is transferred to denaturation, annealing and extension stages of the reaction repeatedly due to thermal convection. Two different modes of fiber-based optical detection are compared, and it shows that the detection sensitivity, repeatability, and consistency can be significantly improved with bottom collection comparing to sidewall collection. Each capillary tube is illuminated by its own LED from the top through a common optical filter, and meanwhile, a camera equipped with another optical filter is adopted to detect the fluorescence signal from optical fibers. To handle the untypical real-time fluorescence curves of negative tests due to non-specific amplification, AI-based classification methods, for example, artificial neural network (ANN) is adopted to improve the detection specificity up to 97.50% comparing to 47.5% or 75.0% which is achieved with Ct-based method. A smartphone is adopted to run the AI algorithm and custom software, as well as to report an ASF event with the geographical information. Experimental results show that with the hand-held CPCR system, within 30 min, ASF viruses can be successfully detected with acceptable sensitivity and specificity.