Pangenomic and functional investigations for dormancy and biodegradation features of an organic pollutant-degrading bacterium Rhodococcus biphenylivorans TG9
Pangenomic and functional investigations for dormancy and biodegradation features of an organic pollutant-degrading bacterium Rhodococcus biphenylivorans TG9
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有机污染物降解细菌联苯红球菌 TG9 休眠和生物降解特征的全基因组和功能研究
DOI：
10.1016/j.scitotenv.2021.151141
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Shen Chaofeng
中科院分区：
文献类型：
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作者：
Yu Chungui;Wang Hui;Blaustein Ryan Andrew;Guo Li;Ye Qi;Fu Yulong;Fan Jiahui;Su Xiaomei;Hartmann Erica Marie;Shen Chaofeng
环境细菌以生物降解基因的形式含有丰富的未开发的潜力。利用这种潜力往往会因为缺乏对基本生存策略的理解而感到困惑，比如休眠，环境压力。调查细菌休眠降解的关系，使生物修复的改善。在这里，我们夫妇的基因组和功能评估提供背景的有机污染物降解菌株Rhodococcus biphenylvoransTG9的关键属性。通过全基因组测序、泛基因组分析和功能鉴定，阐明了红球菌的重要基因和基因产物，包括抗菌剂抗性、休眠和降解。biphenylvoransTG9作为模型。我们确定了四个复苏促进因子（Rpf）编码基因在TG9参与休眠和复苏。我们证明了R. biphenylivoransTG9生长在14种典型的有机污染物上，对联苯和多氯联苯的几种同系物表现出较强的降解能力。我们进一步诱导TG9进入休眠状态，并证明了形态和活性的显著差异。总之，这些结果扩大了我们的理解属Rhodococcus和休眠和生物降解之间的关系，在环境压力的存在。
Environmental bacteria contain a wealth of untapped potential in the form of biodegradative genes. Leveraging this potential can often be confounded by a lack of understanding of fundamental survival strategies, like dormancy, for environmental stress. Investigating bacterial dormancy-to-degradation relationships enables improvement of bioremediation. Here, we couple genomic and functional assessment to provide context for key attributes of the organic pollutant-degrading strainRhodococcus biphenylivoransTG9. Whole genome sequencing, pangenome analysis and functional characterization were performed to elucidate important genes and gene products, including antimicrobial resistance, dormancy, and degradation.Rhodococcusas a genus has strong potential for degradation and dormancy, which we demonstrate usingR. biphenylivoransTG9 as a model. We identified four Resuscitation-promoting factor (Rpf) encoding genes in TG9 involved in dormancy and resuscitation. We demonstrate thatR. biphenylivoransTG9 grows on fourteen typical organic pollutants, and exhibits a robust ability to degrade biphenyl and several congeners of polychlorinated biphenyls. We further induced TG9 into a dormant state and demonstrated pronounced differences in morphology and activity. Together, these results expand our understanding of the genusRhodococcusand the relationship between dormancy and biodegradation in the presence of environmental stressors.