Mechanistic characterization of sulfur transfer from cysteine desulfurase SufS to the iron-sulfur scaffold SufU in Bacillus subtilis
Mechanistic characterization of sulfur transfer from cysteine desulfurase SufS to the iron-sulfur scaffold SufU in Bacillus subtilis
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DOI：
10.1016/j.febslet.2011.01.005
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发表时间：
2011-02-04
期刊：
影响因子：
3.5
通讯作者：
Marahiel, Mohamed A.
中科院分区：
文献类型：
--
作者：
Albrecht, Alexander G.;Peuckert, Florian;Marahiel, Mohamed A.
革兰氏阳性菌中的铁硫簇生物合成是由 SUF 系统介导的。将硫化物从半胱氨酸脱硫酶 SufS 转移到支架蛋白 SufU 是组装过程中的第一步。在本研究中，我们分析了枯草芽孢杆菌 SufS 与其支架 SufU 之间的相互作用。 SufS 的活性代表了一种乒乓机制，导致 SufU 中保守的半胱氨酸残基连续硫负载。 SufU 的半胱氨酸 41 对于从 SufS 接收硫化物至关重要，而半胱氨酸 66 和 128 是 SufS/SufU 相互作用所必需的。总之，我们提出了第一个通过硫供体 SufS 加载基本支架成分 SufU 的分步模型。结构摘要：SufS 和 SufU 通过分子筛结合（查看相互作用）SufS 通过分子筛与 SufS 结合（查看相互作用）SufS 和 SufU 通过酶促研究进行氧化还原反应（查看相互作用 1, 2, 3, 4, 5）SufU 通过下拉与 SufS 发生物理相互作用（查看相互作用 1、2）(C) 2011 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Iron-sulfur cluster biosynthesis in Gram-positive bacteria is mediated by the SUF system. The transfer of sulfide from the cysteine desulfurase SufS to the scaffold protein SufU is one of the first steps within the assembly process. In this study, we analyzed the interaction between Bacillus subtilis SufS and its scaffold SufU. The activity of SufS represents a Ping-Pong mechanism leading to successive sulfur loading of the conserved cysteine residues in SufU. Cysteine 41 of SufU is shown to be essential for receiving sulfide from SufS, while cysteines 66 and 128 are needed for SufS/SufU interaction. In conclusion, we present the first step-by-step model for loading of the essential scaffold component SufU by its sulfur donor SufS.Structured summary:SufS and SufU bind by molecular sieving (View interaction)SufS binds to SufS by molecular sieving (View interaction)SufS and SufU redox react by enzymatic study (View Interaction 1, 2, 3, 4, 5)SufU physically interacts with SufS by pull down (View Interaction 1, 2) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.