Myristoylation-enhanced binding of the HIV-1 Nef protein to T cell skeletal matrix.
Myristoylation-enhanced binding of the HIV-1 Nef protein to T cell skeletal matrix.
复制标题
肉豆蔻酰化增强了 HIV-1 Nef 蛋白与 T 细胞骨骼基质的结合。
DOI：
10.1006/viro.1993.1605
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发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
Ratner,L
中科院分区：
文献类型：
--
作者：
Niederman,TM;Hastings,WR;Ratner,L
HIV-1的负性因子Nef被发现与T淋巴细胞的洗涤剂不溶细胞骨架部分有16%-42%的联系。此外，在大肠杆菌中表达的Nef蛋白在体外反应中与细胞骨架基质的结合程度达到30-50%。肉豆蔻酰化显著增强了Nef的细胞骨架结合。肉豆蔻化增强结合的特异性是由于缺乏肉豆蔻化对HIV-1 Gag蛋白与细胞骨架结合的影响。细胞骨架结合是饱和的，并被高浓度的氯化钠、十二烷基硫酸钠或尿素抑制。Nef与细胞骨架基质的结合在介导其对HIV-1复制的影响方面可能是重要的。
The negative factor, Nef, of HIV-1 was found to associate to an extent of 16-42% with the detergent insoluble cytoskeletal fraction of T lymphocytes. Furthermore,Escherichia coliexpressed Nef protein was found to bind duringin vitroreactions with the cytoskeletal matrix to an extent of 30-50%. Cytoskeletal association of Nef was significantly enhanced by myristoylation. The specificity of the myristoylation-enhanced binding was demonstrated by the lack of an effect of myristoylation on binding of the HIV-1 Gag protein to the cytoskeleton. Cytoskeletal binding was saturable, and inhibited by high concentrations of sodium chloride, or with SDS or urea. Binding of Nef to the cytoskeletal matrix may be important in mediating its effects on HIV-1 replication.