Influence of Evolution in Tumor Biobanking on the Interpretation of Translational Research
Influence of Evolution in Tumor Biobanking on the Interpretation of Translational Research
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DOI：
10.1158/1055-9965.epi-08-0622
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发表时间：
2008-12-01
影响因子：
3.8
通讯作者：
Watson, Peter H.
中科院分区：
文献类型：
--
作者：
Barnes, Rebecca O.;Parisien, Michelle;Watson, Peter H.
目的：转化癌症研究越来越依赖于人类组织生物标本，这与组织生物库方法的转变相吻合。较新的生物库（2000年后）采用标准操作程序，以减少生物标本收集的可变性。由于目前的转化研究是基于前2000年和后2000年时代的生物标本，我们考虑是否收集时代可能会影响基因表达data.Design：我们比较了乳腺肿瘤的收集时间范围从前2000年和后2000年时代的生物银行和比较雌激素受体（ER）蛋白表达与收集时间。然后，我们收集了10个乳腺肿瘤生物标本，在标准化的协议，并检查是否c-myc和ER的表达受到影响，通过在冰上储存
Purpose: Translational cancer research increasingly relies on human tissue biospecimens and this has coincided with a shift in tissue biobanking approach. Newer biobanks (post year 2000) deploy standard operating procedures to reduce variability around biospecimen collection. Because current translational research is based on pre-2000 and post-2000 era biospecimens, we consider whether the collection era may influence gene expression data.Design: We compared the range of breast tumor collection times from pre-2000 and post-2000 era biobanks and compared estrogen receptor (ER) protein expression with collection time. We then collected 10 breast tumor biospecimens under a standardized protocol and examined whether the expression of c-myc and ER was influenced by storage on ice