Reattachment and apoptosis after plasma-needle treatment of cultured cells
Reattachment and apoptosis after plasma-needle treatment of cultured cells
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DOI：
10.1109/tps.2006.876511
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发表时间：
2006-08-01
影响因子：
1.5
通讯作者：
Stoffels, Eva
中科院分区：
文献类型：
--
作者：
Kieft, Ingrid E.;Kurdi, Moamina;Stoffels, Eva
非热等离子体可以用来局部影响细胞黏附：细胞可以从周围移走，而不会造成坏死。事实上，细胞仍然活着，并可以在几个小时内重新附着。这一现象在未来可能被用于显微外科手术。另一种去除细胞的方法是诱导细胞凋亡或程序性细胞死亡。这种类型的细胞死亡比坏死更可取，后者可能会引起炎症反应。当分离的细胞被允许重新附着和生长时，了解它们的状况是很重要的。因此，对等离子针治疗的长期效果进行了评估，特别是对再附着和细胞凋亡的影响。这些细胞被用等离子针处理。这个装置在大气压下产生一个小的(直径1毫米)等离子体。为了避免任何热效应，重要的是等离子体温度等于或低于生理温度。这就是。用于等离子针头的箱子。
Nonthermal plasmas can be used to locally influence cell adhesion: cells can be removed from their surroundings without causing necrosis. In fact, cells remain alive and can reattach within hours. This phenomenon may, in the future, be used for microsurgical procedures. Another method to remove cells is to induce apoptosis or programmed cell death. This type of cell death is preferred above necrosis, which may cause inflammation reactions. When the detached cells are allowed to reattach and grow, it is important to know their condition. Therefore, long-term effects of plasma-needle treatment were assessed, with special focus on reattachment and apoptosis. The cells were treated using a plasma needle. This device generates a small (1-mm diameter) plasma at atmospheric pressure. To avoid any heat effects, it is important that the plasma temperature is at or below physiological temperature. This is the. case for the plasma needle.