EGL‐4/PKG regulates the role of an interneuron in a chemotaxis circuit of C. elegans through mediating integration of sensory signals
EGL‐4/PKG regulates the role of an interneuron in a chemotaxis circuit of C. elegans through mediating integration of sensory signals
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EGL-4/PKG 通过介导感觉信号的整合来调节线虫趋化回路中中间神经元的作用
DOI：
10.1111/gtc.12849
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发表时间：
2021
期刊：
影响因子：
2.1
通讯作者：
Fujiwara Manabi
中科院分区：
文献类型：
--
作者：
Hino Takahiro;Hirai Shota;Ishihara Takeshi;Fujiwara Manabi
中间神经元受多个感觉神经元支配，需要整合来自这些感觉神经元的信息并对感觉刺激做出充分反应。感觉信息如何整合形成中间神经元反应的机制尚未完全了解。在小杆线虫中，编码cGMP依赖性蛋白激酶（PKG）的fegl-4的功能缺失突变导致对气味物的趋化性缺陷。我们的遗传和成像分析显示，AIY中间神经元对气味物的反应特性在egl-4突变体中被逆转，而两个上游嗅觉神经元AWA和AWC的反应基本上没有变化。细胞消融实验表明，egl-4突变体中的AIY具有抑制趋化性的功能。此外，AIY反应的逆转仅在AWA和AWC的感觉信号存在的情况下发生。这些结果表明，感觉信号在egl-4突变体中整合不足。我们还表明，在AWA和另一种感觉神经元中的egl-4表达阻止了逆转的AIY反应，并恢复了egl-4突变体的趋化性。我们认为EGL-4/PKG通过抑制嗅觉神经元信号的异常整合，将中间神经元对嗅觉刺激的反应性质转化为嗅觉刺激，并保持中间神经元在回路中执行趋化行为的作用。
Interneurons, innervated by multiple sensory neurons, need to integrate information from these sensory neurons and respond to sensory stimuli adequately. Mechanisms how sensory information is integrated to form responses of interneurons are not fully understood. InCaenorhabditiselegans, loss‐of‐function mutations ofegl‐4, which encodes a cGMP‐dependent protein kinase (PKG), cause a defect in chemotaxis to odorants. Our genetic and imaging analyses revealed that the response property of AIY interneuron to an odorant is reversed in theegl‐4mutant, while the responses of two upstream olfactory neurons, AWA and AWC, are largely unchanged. Cell‐ ablation experiments show that AIY in theegl‐4mutant functions to suppress chemotaxis. Furthermore, the reversal of AIY response occurs only in the presence of sensory signals from both AWA and AWC. These results suggest that sensory signals are inadequately integrated in theegl‐4mutant. We also show thategl‐4expression in AWA and another sensory neuron prevents the reversed AIY response and restores chemotaxis in theegl‐4mutants. We propose that EGL‐4/PKG, by suppressing aberrant integration of signals from olfactory neurons, converts the response property of an interneuron to olfactory stimuli and maintains the role of the interneuron in the circuit to execute chemotactic behavior.