Ghrelin Receptor Expression in Lymphocytes Isolated from Adult Cystic Fibrosis Patients
Ghrelin Receptor Expression in Lymphocytes Isolated from Adult Cystic Fibrosis Patients
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DOI：
10.1159/000254486
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发表时间：
2010-01-01
期刊：
影响因子：
3.7
通讯作者：
McCloskey, Thomas
中科院分区：
文献类型：
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作者：
Cohen, Rubin I.;Chandra, Subani;McCloskey, Thomas
背景资料：为了探讨囊性纤维化（CF）患者体重减轻的机制，我们研究了不同体重指数（BMI）的CF受试者分离的淋巴细胞上ghrelin受体的表达。进食行为受到激素肽的影响，如生长激素释放肽，一种有效的食欲刺激剂。然而，对CF中ghrelin血浆水平的研究表明，它在恶病质受试者中增加，这是预期的生理反应。目的：（1）比较BMI正常的临床稳定CF患者、恶病质CF患者和健康对照者之间的Ghrelin受体表达。(2)研究急性加重治疗前后同一CF受试者的ghrelin受体表达。方法：从正常BMI和低BMI的CF患者和对照组中分离淋巴细胞。通过流式细胞术测定Ghrelin受体定量。通过生物电阻抗测定身体组成，并测定生长激素释放肽、TNF-α、IL-1和IL-6的血浆水平。结果如下：低BMI的CF受试者炎症增加，血浆细胞因子增加，淋巴细胞生长激素释放肽受体表达降低。在具有正常BMI的CF组中，Ghrelin受体表达与对照组相似;在与体重减轻相关的急性加重期间，Ghrelin受体表达降低，并在治疗和体重减轻恢复后恢复至基线。结论：不同BMI的稳定期CF患者外周血淋巴细胞Ghrelin受体表达存在差异。这些变化可能是由于对体重减轻的紊乱病理反应。版权所有（C）2009 S. Karger AG，巴塞尔
Background: To explore mechanisms of weight loss in cystic fibrosis (CF), we studied ghrelin receptor expression on isolated lymphocytes from CF subjects with different body mass indices (BMIs). Eating behavior is influenced by hormone peptides such as ghrelin, a potent appetite stimulator. However, studies on ghrelin plasma levels in CF showed it to be increased in cachectic subjects, the expected physiological response. Objectives: (1) To compare ghrelin receptor expression between clinically stable CF subjects with normal BMI, CF subjects with cachexia and healthy controls. (2) To investigate ghrelin receptor expression in the same CF subjects before and after treatment for an acute exacerbation. Methods: Lymphocytes were isolated from CF patients with normal BMI and low BMI and from controls. Ghrelin receptor quantification was determined via flow cytometry. Body composition was determined by bioelectrical impedance, and plasma levels of ghrelin, TNF-alpha, IL-1 and IL-6 were determined. Results: CF subjects with low BMI had increased inflammation evidenced by increased plasma cytokines and showed decreased lymphocytic ghrelin receptor expression. Ghrelin receptor expression in the CF group with normal BMI was similar to controls; it decreased during an acute exacerbation associated with weight loss and returned to baseline following treatment and recovery of the weight loss. Conclusions: Differences exist in ghrelin receptor expression in lymphocytes isolated from stable CF patients with different BMIs. These changes may be due to a disordered pathological response to weight loss. Copyright (C) 2009 S. Karger AG, Basel