Cloning, functional expression and tissue distribution of human α 1C‐adrenoceptor splice variants
Cloning, functional expression and tissue distribution of human α
1C‐adrenoceptor splice variants
复制标题
人α的克隆、功能表达及组织分布
DOI：
10.1016/0014-5793(95)00330-c
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发表时间：
1995
期刊：
影响因子：
3.5
通讯作者：
G. Tsujimoto
中科院分区：
文献类型：
--
作者：
A. Hirasawa;K. Shibata;K. Horie;Y. Takei;K. Obika;Teruo Tanaka;Noriyuki Muramoto;K. Takagaki;J. Yano;G. Tsujimoto
我们报道了人α1C-肾上腺素受体基因的两个亚型(α1C-2和αC-3)的克隆和鉴定。这些异构体是通过选择性剪接产生的，在长度和C-末端结构域的序列方面与我们先前分离的克隆(α1C-1)不同。组织分布结果显示，这些变异体与α1C-1在人的心脏、肝脏、小脑和大脑中共表达。尽管结构上存在差异，但在转基因CHO细胞中的功能实验表明，这三种异构体具有相似的配体结合特性，并且都与磷脂酶C/Ca~(2+)信号通路相连。
We report the cloning and characterization of two isoforms of humanα1C‐adrenoceptor cDNA (α1C‐2,αC‐3). These isoforms are generated by alternative splicing and differ from the clone we previously isolated (α1C‐1) in their length and sequences of the C‐terminal domain. Tissue distribution of mRNAs showed that these variants co‐express withα1C‐1 in the human heart, liver, cerebellum and cerebrum. Despite the structural differences, functional experiments in transfected CHO cells showed that the three isoforms have similar ligand binding properties, and all couple with phospholipase C/Ca2+signaling pathway.