Functional Diversity of Glycinergic Commissural Inhibitory Neurons in Larval Zebrafish
Functional Diversity of Glycinergic Commissural Inhibitory Neurons in Larval Zebrafish
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DOI：
10.1016/j.celrep.2020.02.015
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发表时间：
2020-03-03
期刊：
影响因子：
8.8
通讯作者：
Higashijima, Shin-ichi
中科院分区：
文献类型：
--
作者：
Satou, Chie;Sugioka, Takumi;Higashijima, Shin-ichi
水生脊椎动物脊髓中的连合抑制神经元在游泳过程中协调左右身体交替。然而，它们的发展起源一直难以捉摸。我们调查这两个连合抑制神经元类型，dl6dmrt3a和V0d，分别来自pd6和p0祖域的解剖和功能进行比较。我们发现，这两种连合神经元类型具有单突触，抑制连接的神经元群体活跃在虚构的游泳，支持他们的作用，提供抑制对侧。V0d神经元倾向于在更快和更强的运动中放电，而dl6dmrt3a神经元倾向于在正常的虚构游泳中更一致地放电。dl6dmrt3a神经元的消融通过脊髓两侧腹根神经元的异常共激活导致左右交替活动的损伤。我们的研究结果表明，dl6dmrt3a和V0d连合抑制神经元协同提供抑制对不同的游泳行为的对侧。
Commissural inhibitory neurons in the spinal cord of aquatic vertebrates coordinate left-right body alternation during swimming. Their developmental origin, however, has been elusive. We investigate this by comparing the anatomy and function of two commissural inhibitory neuron types, dl6dmrt3a and V0d, derived from the pd6 and p0 progenitor domains, respectively. We find that both of these commissural neuron types have monosynaptic, inhibitory connections to neuronal populations active during fictive swimming, supporting their role in providing inhibition to the contralateral side. V0d neurons tend to fire during faster and stronger movements, while dl6dmrt3a neurons tend to fire more consistently during normal fictive swimming. Ablation of dl6dmrt3a neurons leads to an impairment of left-right alternating activity through abnormal co-activation of ventral root neurons on both sides of the spinal cord. Our results suggest that dl6dmrt3a and V0d commissural inhibitory neurons synergistically provide inhibition to the opposite side across different swimming behaviors.