Allergen-specific immune response suppresses interleukin 10 expression in B cells via increasing micro-RNA-17-92 cluster.
Allergen-specific immune response suppresses interleukin 10 expression in B cells via increasing micro-RNA-17-92 cluster.
复制标题
过敏原特异性免疫反应通过增加 micro-RNA-17-92 簇来抑制 B 细胞中白细胞介素 10 的表达。
DOI：
10.1002/cbf.3207
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发表时间：
2016
影响因子：
3.6
通讯作者：
Yang Ping-Chang
中科院分区：
文献类型：
--
作者：
Geng Xiao-Rui;Qiu Shu-Qi;Yang Li-Tao;Liu Zhi-Qiang;Yang Gui;Liu Jiang-Qi;Zeng Lu;Li Xiao-Xi;Mo Li-Hua;Liu Zhi-Gang;Yang Ping-Chang
表达白介素10的B细胞在体内的免疫稳态中起着关键作用，其调节机制尚未完全清楚。Micro-RNA(MiR)-17-92簇具有较强的免疫调节作用。这项研究验证了miR-17-92簇抑制B细胞IL-10表达的假设。本研究收集变应性鼻炎(AR)患者外周血中的B细胞。B细胞在培养中用特定的过敏原--尘螨提取物处理。用实时定量逆转录聚合酶链式反应和酶联免疫吸附试验检测细胞中miR-17-92簇和IL-10的表达。结果显示，AR患者外周血B细胞中miR-19a的表达水平显著高于正常人B细胞，而其余5个成员(miR-17、miR-18a、miR-19b、miR-20a和miR-92a)的表达水平均显著高于正常人。AR患者的B细胞暴露于特定的过敏原、尘螨提取物后，可显著增加B细胞中miR-19a的水平，并抑制B细胞中IL-10的表达。在IL-10启动子区，组蛋白脱乙酰酶11和乙酰化H3K9水平较高，而RNA聚合酶II和c-Maf(IL-10转录因子)水平较低。综上所述，miR-19a介导了变应原特异性免疫反应--B细胞IL-10表达降低。
Interleukin (IL)‐10–expressing B cells play a critical role in the immune homeostasis in the body; its regulation has not been fully understood. Micro‐RNA (miR)‐17‐92 cluster has strong regulation in the immunity. This study tests a hypothesis that miR‐17‐92 cluster suppresses IL‐10 expression in B cells. In this study, peripheral B cells were collected from patients with allergic rhinitis (AR). The B cells were treated with specific allergens, dust mite extracts, in the culture. The expressions of miR‐17‐92 cluster and IL‐10 in the culture were assessed by real‐time quantitative reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay. The results showed that the levels of miR‐19a, but not the rest of the 5 members (miR‐17, miR‐18a, miR‐19b, miR‐20a, and miR‐92a), were significantly higher in peripheral B cells from AR patients as than in B cells from healthy participants. Exposure of B cells from AR patients to specific allergen, dust mite extracts, significantly increased the levels if miR‐19a and suppressed the expression of IL‐10 in B cells. The levels of histone deacetylase 11 and acetylated H3K9 were higher, and the RNA polymerase II and c‐Maf (the IL‐10 transcription factor) were lower, at the IL‐10 promoter locus. In conclusion, miR‐19a mediates the allergen‐specific immune response–decreased IL‐10 expression in B cells.