Simultaneous determination of cyanide and thiocyanate in plasma by chemical ionization gas chromatography mass-spectrometry (CI-GC-MS)
Simultaneous determination of cyanide and thiocyanate in plasma by chemical ionization gas chromatography mass-spectrometry (CI-GC-MS)
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DOI：
10.1007/s00216-012-6360-5
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发表时间：
2012-11-01
影响因子：
4.3
通讯作者：
Logue, Brian A.
中科院分区：
文献类型：
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作者：
Bhandari, Raj K.;Oda, Robert P.;Logue, Brian A.
建立了化学电离气相色谱-质谱法同时测定血浆中氰化物和硫氰酸盐的分析方法。用于该分析的样品制备基本上需要一个步骤，通过将氰化物和硫氰酸盐与五氟苄基溴的反应结合，并同时将产物萃取到乙酸乙酯中，通过相转移催化剂四丁基硫酸铵来促进。氰化物和硫氰酸盐的检测限分别为1 μ M和50 nM。线性动态范围是从10 μ M到20 mM的氰化物和从500 nM到200 μ M的硫氰酸盐与氰化物和硫氰酸盐的相关系数高于0.999。对于分析的所有质控标准品，精密度（通过%RSD测定）低于9%，准确度在标称浓度的15%范围内。血浆中氰化物和硫氰酸盐的总回收率均在90%以上。利用该方法，对猪血浆中氰化物和硫氰酸盐的毒代动力学行为进行了研究。
An analytical method utilizing chemical ionization gas chromatography-mass spectrometry was developed for the simultaneous determination of cyanide and thiocyanate in plasma. Sample preparation for this analysis required essentially one-step by combining the reaction of cyanide and thiocyanate with pentafluorobenzyl bromide and simultaneous extraction of the product into ethyl acetate facilitated by a phase-transfer catalyst, tetrabutylammonium sulfate. The limits of detection for cyanide and thiocyanate were 1 mu M and 50 nM, respectively. The linear dynamic range was from 10 mu M to 20 mM for cyanide and from 500 nM to 200 mu M for thiocyanate with correlation coefficients higher than 0.999 for both cyanide and thiocyanate. The precision, as measured by %RSD, was below 9 %, and the accuracy was within 15 % of the nominal concentration for all quality control standards analyzed. The gross recoveries of cyanide and thiocyanate from plasma were over 90 %. Using this method, the toxicokinetic behavior of cyanide and thiocyanate in swine plasma was assessed following cyanide exposure.