Structural diversity requires individual optimization of ethanol concentration in polysaccharide precipitation
Structural diversity requires individual optimization of ethanol concentration in polysaccharide precipitation
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DOI：
10.1016/j.ijbiomac.2014.03.036
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发表时间：
2014-06-01
影响因子：
8.2
通讯作者：
Han, Quan-Bin
中科院分区：
文献类型：
--
作者：
Xu, Jun;Yue, Rui-Qi;Han, Quan-Bin
乙醇沉淀法是制备天然多糖最常用的方法之一，其中乙醇浓度显著影响沉淀产率，然而，通常设定为70- 80%。乙醇浓度的标准化是否合适尚未研究。在本研究中，在不同的乙醇浓度（10-90%）产生的沉淀产量进行了定性和定量的HPGPC（高效凝胶渗透色谱法），使用两个系列的标准葡聚糖，即葡聚糖和支链淀粉，作为参考样品，然后8个天然样品。结果表明，响应的多糖的化学结构，具有多样性的结构特征和分子大小，乙醇浓度是这些葡聚糖沉淀的决定性因素。具有不同结构特征的多糖，即使它们具有相似的分子量，也表现出显著不同的沉淀行为。对于特定的葡聚糖，其分子大小越小，完全沉淀所需的乙醇浓度越高。当分子大小从1 kDa增加到270 kDa时，在80%乙醇中的沉淀产率从10%变化到100%。本文旨在引起科学家们的注意，在乙醇沉淀法提取天然多糖时，必须针对每种类型的材料单独优化乙醇浓度。(C)2014爱思唯尔有限公司版权所有。
Ethanol precipitation is one of the most widely used methods for preparing natural polysaccharides, in which ethanol concentration significantly affects the precipitate yield, however, is usually set at 70-80%. Whether the standardization of ethanol concentration is appropriate has not been investigated. In the present study, the precipitation yields produced in varied ethanol concentrations (10-90%) were qualitatively and quantitatively evaluated by HPGPC (high-performance gel-permeation chromatography), using two series of standard glucans, namely dextrans and pullulans, as reference samples, and then eight natural samples. The results indicated that the response of a polysaccharide's chemical structure, with diversity in structural features and molecular sizes, to ethanol concentration is the decisive factor in precipitation of these glucans. Polysaccharides with different structural features, even though they have similar molecular weights, exhibit significantly different precipitation behaviors. For a specific glucan, the lower its molecular size, the higher the ethanol concentration needed for complete precipitation. The precipitate yield varied from 10% to 100% in 80% ethanol as the molecular size increased from 1 kDa to 270 kDa. This paper aims to draw scientists' attention to the fact that, in extracting natural polysaccharides by ethanol precipitation, the ethanol concentration must be individually optimized for each type of material. (C) 2014 Elsevier B.V. All rights reserved.