Efficient post-exposure prophylaxis against rabies by applying a four-dose DNA vaccine intranasally
Efficient post-exposure prophylaxis against rabies by applying a four-dose DNA vaccine intranasally
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DOI：
10.1016/j.vaccine.2008.09.083
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发表时间：
2008-12-09
期刊：
影响因子：
5.5
通讯作者：
Aguilar Setien, Jose Alvaro
中科院分区：
文献类型：
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作者：
Tesoro Cruz, Emiliano;Feria Romero, Iris Angelica;Aguilar Setien, Jose Alvaro
我们在实验动物身上测试了两种狂犬病暴露后预防(PEP)：一种是传统的人用抗狂犬病疫苗肌肉注射(IM)，另一种是DNA疫苗鼻腔注射(IN)。与世界卫生组织推荐的五剂PEP形成鲜明对比。我们只给了四个没有超免疫抗狂犬病血清的封闭剂，使PEP更加严格。所有动物均用攻击病毒株(CVS)攻击：16h后，注射PEP。接受带有DNA/IN的PEP的所有动物存活，接受带有传统抗狂犬病疫苗/IM的PEP的87%的兔子和80%的小鼠存活。阴性对照组死于感染。G蛋白在NALT、小脑、大脑皮层(新皮质)、小脑和海马区均有表达，主要分布在胶质细胞(小胶质细胞)和微血管中。另一方面，在脑内检测到了质粒构建，并在中脑和后脑中检测到了其mRNA的表达。这种DNA/IN PEP的有效性可能是由于这种抗原在大脑中的早期表达，刺激了局部的免疫系统。(C)2008爱思唯尔有限公司。保留所有权利。
We tested two post-exposure prophylaxes (PEPs) for rabies in laboratory animals: one was a traditional antirabies vaccine for-humans via intramuscular route (IM), and the other was a DNA vaccine administered by intranasal route (IN). in contrast to The World Health Organization's recommended five-dose PEP. we gave only four closes without hyper-immune antirabies sera, making the PEP more rigorous. All animals were challenged with challenge virus strain (CVS): 16 h later, PEP was applied. All animals that received the PEP with DNA/IN survived, and 87% of the rabbits and 80% of the mice that received the PEP with traditional antirabies vaccine/IM survived. Negative controls succumbed to infection. The expression of G protein was detected in the NALT, cerebellum, cerebral cortex (neocortex), cerebellum and hippocampus, mainly in the glial cells (microglia) and microvessels. On the other hand, plasmid construct was detected in brain and its mRNA expression in medium and posterior encephalon. The efficiency of this DNA/IN PEP is probably due to the early expression of the antigen in the brain stimulating the immune system locally. (C) 2008 Elsevier Ltd. All rights reserved.