Characterization of Propionibacterium acnes isolates from sarcoid and non-sarcoid tissues with special reference to cell invasiveness, serotype, and trigger factor gene polymorphism
Characterization of Propionibacterium acnes isolates from sarcoid and non-sarcoid tissues with special reference to cell invasiveness, serotype, and trigger factor gene polymorphism
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DOI：
10.1016/j.micpath.2008.10.013
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发表时间：
2009-02-01
影响因子：
3.8
通讯作者：
Eishi, Yoshinobu
中科院分区：
文献类型：
--
作者：
Furukawa, Asuka;Uchida, Keisuke;Eishi, Yoshinobu
结节病是一种病因不明的全身性肉芽肿性疾病。痤疮丙酸杆菌是迄今为止唯一从结节病皮损中分离到的微生物。为了检验从结节病组织分离的痤疮丙酸杆菌是否与从非结节病组织获得的痤疮丙酸杆菌不同，我们研究了从结节病淋巴结分离的35株痤疮丙酸杆菌和从非结节病组织分离的127株痤疮丙酸杆菌的细胞侵袭性、血清型和痤疮丙酸杆菌触发因子蛋白和两种侵袭相关蛋白（命名为PAmce和PAp 60）的多态性。大多数血清型I分离株（79/112; 71%），但血清型11分离株（0/50）均为细胞侵袭性。两个突出类型的触发因子，一个具有和一个不具有15个氨基酸残基缺失，分别对应于血清型11和血清型1。非侵入性分离株的基因组突变导致PAmce或PAp 60基因中一个以上的氨基酸变化，其中有四个例外的分离株。痤疮杆菌最终被分为9个同种型，从结节病和非结节病组织中获得的分离株没有差异。虽然这一发现没有将痤疮丙酸杆菌与结节病联系起来，但本研究阐明了痤疮丙酸杆菌的细胞侵袭性以及细胞侵袭性与两种侵袭相关的痤疮丙酸杆菌基因的血清型和基因型的密切相关性。(C)2008爱思唯尔有限公司保留所有权利。
Sarcoidosis is a systemic granulomatous disease of unknown etiology. Propionibacterium acnes is the only microorganism so far isolated from sarcoid lesions. To examine whether P. acnes isolates from sarcoid tissues differ from those obtained from non-sarcoid tissues, we studied cell invasiveness, serotype, and polymorphisms of the P. acnes trigger factor protein and the two invasion-associated proteins (named PAmce and PAp60) in 35 P. acnes isolates from sarcoid lymph nodes and 127 isolates from non-sarcoid tissues. Most of the serotype I isolates (79/112; 71%), but none of the serotype 11 isolates (0/50) were cell-invasive. Two prominent types of trigger factors, one with and one without a 15 amino acid-residue deletion, corresponded to serotype 11 and serotype 1, respectively. Non-invasive isolates had genomic mutations that caused more than one amino acid change in either the PAmce or PAp60 gene, with four exceptional isolates. R acnes was finally classified into nine isotypes, and isolates obtained from sarcoid and non-sarcoid tissue did not differ. Although the finding did not link P. acnes to sarcoidosis, the present study clarified the cell invasiveness of R acnes and the close correlation of cell invasiveness to the serotype and genotype of the two invasion-associated P. acnes genes. (C) 2008 Elsevier Ltd. All rights reserved.