AN UNUSUAL SPLIT DROSOPHILA HEAT-SHOCK GENE EXPRESSED DURING EMBRYOGENESIS, PUPATION AND IN TESTIS
AN UNUSUAL SPLIT DROSOPHILA HEAT-SHOCK GENE EXPRESSED DURING EMBRYOGENESIS, PUPATION AND IN TESTIS
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DOI：
10.1016/0022-2836(88)90332-4
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发表时间：
1988-03-05
影响因子：
5.6
通讯作者：
AYMESOUTHGATE, A
中科院分区：
文献类型：
--
作者：
PAULI, D;TONKA, CH;AYMESOUTHGATE, A
基因2是来自黑腹果蝇67B位点的7个热休克基因之一，被转录成两个具有不同发育表达谱的聚腺苷化rna。较小的转录本大约有560个核苷酸，从中期到前两个幼虫阶段和在化蛹开始时再次表达。更大的转录本，780个核苷酸，包含一个额外的5”外显子，说明它的大小更大。它在蛹和成虫中被发现，是男性特有的，并且局限于睾丸。热休克不影响这两种转录本的丰度，但诱导约2000个核苷酸的第三种RNA的积累。这种热休克RNA与胚胎转录物具有相同的帽位，而其3‘’部分完全包括邻近的hsp22基因。因此，在这种情况下，热休克似乎改变了正常的转录终止过程。与大多数热休克基因相比，基因2含有几个微内含子。三个转录本共有的一个长开放阅读框编码111个氨基酸残基的推定多肽。与67B位点其他热休克小基因无同源性。
Gene 2, one of the seven heat shock genes from locus 67B of Drosophila melanogaster, is transcribed into two polyadenylated RNAs having different developmental profiles of expression. The smaller transcript, of about 560 nucleotides, is expressed from midembryogenesis to the first two larval stages and again at the beginning of pupation. The larger transcript, 780 nucleotides, contains an additional 5'' exon, accounting for its larger size. It is detected in pupae and adults, is male-specific and is localized in the testes. Heat shock does not affect the abundance of these two transcripts but induces the accumulation of a third RNA species of about 2000 nucleotides. This heat-shock RNA has the same cap site as the embryonic transcript, while its 3'' portion entirely includes the neighbouring hsp22 gene. It appears, therefore, that in this case, heat shock alters the normal transcription termination process. By contrast to most heat shock genes, gene 2 contains several micro introns. One long open reading frame common to the three transcripts encodes a putative polypeptide of 111 amino acid residues. No homology was found with the other small heat shock genes of locus 67B.