Amphiphilic silicones to reduce the absorption of small hydrophobic molecules
Amphiphilic silicones to reduce the absorption of small hydrophobic molecules
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DOI：
10.1016/j.actbio.2020.11.041
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发表时间：
2021-01-27
影响因子：
9.7
通讯作者：
Domenech, Maribella
中科院分区：
文献类型：
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作者：
Quinones-Perez, Manuel;Cieza, Ruben J.;Domenech, Maribella
硅酮（即交联聚（二甲基硅氧烷），PDMS）是用于微流体装置制造的常用材料。尽管如此，由于小的疏水分子（<lkDa）不可控制地吸收到本体中，其对基于细胞的药物测定和传感应用的适用性受到限制。在这里，我们证明了使用的基板的有机硅散装改性与聚（环氧乙烷）硅烷两亲物（PEO-SA），以减少疏水性小分子螯合细胞为基础的测定。以2重量%的浓度产生改性的有机硅基底，9wt.%和14重量% PEO-SA。除了水接触角分析以评估表面疏水性之外，还通过FTIR分析来评估PEO-SA并入到硅酮本体中。还评价了细胞毒性、小疏水性药物的吸收和细胞对疏水性分子的反应。结果表明，将PEO-SA掺入到有机硅中导致水接触角从114度降低到低至16度，并稳定至少三个月。改性硅胶显示NIH-3 T3、MCF 7、MDA-MB-468和MDA-MB-231细胞系的活力值高于85%。使用他莫昔芬和MCF 7细胞系的药物反应测定显示，当暴露于用9 wt.%或14重量% PEO-SA与组织培养塑料相比。因此，我们的研究支持使用浓度为9重量%的PEO-SA。或更高，以增强表面润湿性并减少PDMS基平台中小疏水分子的吸收。聚二甲基硅氧烷（PDMS）是微流控器件制造中常用的材料，但小的疏水分子的不可控制的吸收，（< 1 kDa）进入有机硅本体中，限制了它们在水溶液中的药物测定和疏水传感应用中的适用性。本研究探讨了PDMS与聚环氧乙烷硅烷两亲物（PEO-SA）结合的亲水性和细胞相容性。所示数据支持使用浓度为9重量%的PEO-SA。或更高，用于增强和稳定的表面润湿性，并减少小疏水分子在硅中的吸收，以用于基于细胞的和疏水的取样应用。(c)2020 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Silicones (i.e. crosslinked poly(dimethylsiloxane), PDMS) are commonly used material for microfluidic device fabrication. Nonetheless, due to the uncontrollable absorption of small hydrophobic molecules (< 1 kDa) into the bulk, its applicability to cell-based drug assays and sensing applications has been limited. Here, we demonstrate the use of substrates made of silicones bulk modified with a poly(ethylene oxide) silane amphiphile (PEO-SA) to reduce hydrophobic small molecule sequestration for cell-based assays. Modified silicone substrates were generated with concentrations of 2 wt.%, 9 wt.% and, 14 wt.% PEO-SA. Incorporation of PEO-SA into the silicone bulk was assessed by FTIR analysis in addition to water contact angle analysis to evaluate surface hydrophobicity. Cell toxicity, absorption of small hydrophobic drugs, and cell response to hydrophobic molecules were also evaluated. Results showed that the incorporation of the PEO-SA into the silicone led to a reduction in water contact angle from 114 degrees to as low as 16 degrees that was stable for at least three months. The modified silicones showed viability values above 85% for NIH-3T3, MCF7, MDA-MB-468, and MDA-MB-231 cell lines. A drug response assay using tamoxifen and the MCF7 cell line showed full recovery of cell toxicity response when exposed to PDMS modified with 9 wt.% or 14 wt.% PEO-SA compared to tissue culture plastic. Therefore, our study supports the use of PEO-SA at concentrations of 9 wt.% or higher for enhanced surface wettability and reduced absorption of small hydrophobic molecules in PDMS-based platforms.Statement of significance Silicones, such as poly(dimethylsiloxane) known as PDMS, are commonly used material for microfluidic device fabrication, yet the uncontrollable absorption of small hydrophobic molecules ( < 1 kDa) into the bulk of silicones, limit their applicability into drug assays and hydrophobic sensing applications in aqueous solutions. The present study examined the hydrophilic properties and cell compatibility of PDMS combined with poly(ethylene oxide) silane amphiphile (PEO-SA). The data shown supports the use of PEO-SA at concentrations of 9 wt.% or higher for enhanced and stable surface wettability and reduced absorption of small hydrophobic molecules in silicons for cell-based and hydrophobic sampling applications. (c) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.