Interferon-gamma and sinusoidal electric fields signal by modulating NAD(P)H oscillations in polarized neutrophils.
Interferon-gamma and sinusoidal electric fields signal by modulating NAD(P)H oscillations in polarized neutrophils.
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通过调节极化中性粒细胞中的 NAD(P)H 振荡来发出干扰素 γ 和正弦电场信号。
DOI：
10.1016/s0006-3495(00)76536-2
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Petty,HR
中科院分区：
文献类型：
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作者：
Rosenspire,AJ;Kindzelskii,AL;Petty,HR
已知真核细胞的代谢活动会自然振荡。我们最近在中性粒细胞和其他极化细胞中观察到 20 秒周期的 NAD(P)H 振荡。在这里，我们表明，当极化的人类中性粒细胞暴露于干扰素-γ或周期为 NAD(P)H 振荡周期两倍的超低频电场时，NAD(P)H 振荡的幅度会增加。此外，NAD(P)H 振幅的增加，无论是由干扰素-γ介导还是由振荡电场介导，都表明活性氧代谢物的产生增加。因此，NAD(P)H 振荡的幅度调节表明极化细胞中存在一种新的信号传导机制。
Metabolic activity in eukaryotic cells is known to naturally oscillate. We have recently observed a 20-s period NAD(P)H oscillation in neutrophils and other polarized cells. Here we show that when polarized human neutrophils are exposed to interferon-γor to ultra-low-frequency electric fields with periods double that of the NAD(P)H oscillation, the amplitude of the NAD(P)H oscillations increases. Furthermore, increases in NAD(P)H amplitude, whether mediated by interferon-γor by an oscillating electric field, signals increased production of reactive oxygen metabolites. Hence, amplitude modulation of NAD(P)H oscillations suggests a novel signaling mechanism in polarized cells.