OSMAC approach leads to new fusarielin metabolites from Fusarium tricinctum
OSMAC approach leads to new fusarielin metabolites from Fusarium tricinctum
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DOI：
10.1038/ja.2017.21
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发表时间：
2017-06-01
影响因子：
3.3
通讯作者：
Proksch, Peter
中科院分区：
文献类型：
--
作者：
Hemphill, Catalina F. Perez;Sureechatchaiyan, Parichat;Proksch, Peter
使用OSMAC（单菌株MAny化合物）方法，在水果和蔬菜汁补充的固体大米培养基上培养真菌内生真菌三环镰刀菌。这导致新的天然产物镰孢菌素J（1）的积累增加高达80倍，以及诱导两种新的天然产物镰孢菌素K（2）和镰孢菌素L（3）以及已知的衍生物镰孢菌素A（4）和B（5）。当真菌生长在缺乏水果或蔬菜汁的大米培养基上时，未检测到化合物2-5。在苹果和胡萝卜汁的存在下观察到化合物1的积累的最高增加，而香蕉汁的刺激作用较弱。化合物1对人卵巢癌细胞系A2780表现出细胞毒性，IC 50值为12.5 μ m。
Using the OSMAC (One Strain MAny Compounds) approach, the fungal endophyte Fusarium tricinctum was cultivated on fruit and vegetable juice-supplemented solid rice media. This led to an up to 80-fold increase in the accumulation of the new natural product fusarielin J (1), as well as to the induction of two new natural products fusarielin K (2) and fusarielin L (3) and the known derivatives fusarielins A (4) and B (5). Compounds 2-5 were not detected when the fungus was grown on rice media lacking either fruit or vegetable juice. The highest increase in the accumulation of compound 1 was observed in the presence of apple and carrot juice, whereas the stimulating effect was weaker for banana juice. Compound 1 exhibited cytotoxicity against the human ovarian cancer cell line A2780, with an IC50 value of 12.5 mu m.