Role of synthetic extracellular matrix in development of engineered dental pulp
Role of synthetic extracellular matrix in development of engineered dental pulp
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DOI：
10.1163/156856298x00127
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发表时间：
1998-01-01
影响因子：
3.6
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Bohl, KS;Shon, J;Mooney, DJ
在口腔组织受损的情况下，传统的治疗方法，如根管治疗，用合成材料代替受伤的组织。然而，虽然目前使用的材料可以提供丢失组织的结构替代，但它们不能完全替代原始组织的功能，并且经常随着时间的推移而失效。本报告描述了一种组织工程的方法，牙髓组织替代利用培养细胞接种在合成细胞外基质。获得人牙髓成纤维细胞并在培养物中增殖。然后将这些细胞接种到三种不同的合成基质上：由聚乙醇酸（PGA）纤维，I型胶原蛋白水凝胶和藻酸盐制成的支架，以研究哪种基质最适合牙髓组织形成。此外，以前开发的接种和培养纸浆细胞PGA的方法进行了优化。在PGA上培养细胞导致具有显著胶原沉积的非常高的细胞密度组织。在藻酸盐上没有观察到细胞增殖，45天后胶原凝胶中的细胞生长仅为中等。这些研究表明，牙髓样组织可以被工程化，这可能是工程化完整牙齿的第一步。
In cases of damaged oral tissues, traditional therapies, such as a root canal, replace the injured tissue with a synthetic material. However, while the materials currently used can offer structural replacement of the lost tissue, they are incapable of completely replacing the function of the original tissue, and often fail over time. This report describes a tissue engineering approach to dental pulp tissue replacement utilizing cultured cells seeded upon synthetic extracellular matrices. Human pulp fibroblasts were obtained and multiplied in culture. These cells were then seeded onto three different synthetic matrices: scaffolds fabricated from polyglycolic acid (PGA) fibers, a type I collagen hydrogel, and alginate in an effort to examine which matrix is most suitable for dental pulp tissue formation. In addition, methods previously developed for seeding and culturing pulp cells on PGA were optimized. Culturing cells on PGA resulted in a very high cell density tissue with significant collagen deposition. No cell proliferation was observed on alginate, and the growth of cells in collagen gels after 45 days was only moderate. These studies indicate dental pulp-like tissues can be engineered, and this may provide the first step to engineering a complete tooth.