Identification of QTL and underlying genes for root system architecture associated with nitrate nutrition in hexaploid wheat
Identification of QTL and underlying genes for root system architecture associated with nitrate nutrition in hexaploid wheat
复制标题
DOI：
10.1016/s2095-3119(21)63700-0
复制
发表时间：
2022-03-15
影响因子：
4.8
通讯作者：
Wells, Darren M.
中科院分区：
文献类型：
--
作者：
Griffiths, Marcus;Atkinson, Jonathan A.;Wells, Darren M.
作物的根系构型对养分的吸收和潜在产量有着深远的影响。然而，很少有人知道RSA的遗传基础和资源适应性反应在小麦（Triticum aestivum L.）。在这里，一个高通量的发芽纸为基础的植物表型系统被用来确定小麦双单倍体作图群体，SavannahxRialto的幼苗性状。在整个群体中发现了显着的基因型和硝态氮处理变异的幼苗性状与不同的性状分组根大小相关的性状和根分布相关的性状。QTL分析共检测到59个苗期性状QTL。在两个硝态氮处理中，有27个根系QTL对硝态氮处理具有特异性。对染色体2D上的一个QTL进行转录组学分析，发现在低硝酸盐条件下，基因富集在N相关的生物过程和28个差异表达的基因可能参与根角反应。总之，这些发现提供了对根系结构和植物对硝酸盐的适应性反应的遗传见解，以及可以帮助提高小麦氮捕获的目标。
The root system architecture (RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive responses in wheat (Triticum aestivum L.). Here, a high-throughput germination paper-based plant phenotyping system was used to identify seedling traits in a wheat doubled haploid mapping population, SavannahxRialto. Significant genotypic and nitrate-N treatment variation was found across the population for seedling traits with distinct trait grouping for root size-related traits and root distribution-related traits. Quantitative trait locus (QTL) analysis identified a total of 59 seedling trait QTLs. Across two nitrate treatments, 27 root QTLs were specific to the nitrate treatment. Transcriptomic analyses for one of the QTLs on chromosome 2D, which was found under low nitrate conditions, revealed gene enrichment in N-related biological processes and 28 differentially expressed genes with possible involvement in a root angle response. Together, these findings provide genetic insight into root system architecture and plant adaptive responses to nitrate, as well as targets that could help improve N capture in wheat.