Pre-adsorption of antibodies enables targeting of nanocarriers despite a biomolecular corona
Pre-adsorption of antibodies enables targeting of nanocarriers despite a biomolecular corona
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DOI：
10.1038/s41565-018-0171-6
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发表时间：
2018-09-01
影响因子：
38.3
通讯作者：
Mailaender, Volker
中科院分区：
文献类型：
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作者：
Tonigold, Manuel;Simon, Johanna;Mailaender, Volker
为了促进药物递送到精确的位点和细胞类型，纳米载体的表面用靶向抗体或配体进行功能化，通常通过共价化学偶联。然而，一旦纳米载体暴露于血浆等生物液体中，其表面不可避免地会被各种生物分子覆盖，形成蛋白质冠，从而掩盖了纳米颗粒的靶向能力。在这里，我们表明我们可以使用预吸附过程将靶向抗体附着到纳米载体的表面。预吸附的抗体保持功能，并且不完全被生物分子冠交换或覆盖，而偶联的抗体更容易受到这种屏蔽的影响。我们得出的结论是，预吸附可能是一种将靶向部分附着到纳米载体表面的通用、有效和快速的方法。
To promote drug delivery to exact sites and cell types, the surface of nanocarriers is functionalized with targeting antibodies or ligands, typically coupled by covalent chemistry. Once the nanocarrier is exposed to biological fluid such as plasma, however, its surface is inevitably covered with various biomolecules forming the protein corona, which masks the targeting ability of the nanoparticle. Here, we show that we can use a pre-adsorption process to attach targeting antibodies to the surface of the nanocarrier. Pre-adsorbed antibodies remain functional and are not completely exchanged or covered by the biomolecular corona, whereas coupled antibodies are more affected by this shielding. We conclude that pre-adsorption is potentially a versatile, efficient and rapid method of attaching targeting moieties to the surface of nanocarriers.