Photodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiation
Photodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiation
复制标题
DOI：
10.1007/s10295-008-0446-2
复制
发表时间：
2008-11-01
影响因子：
3.4
通讯作者：
Almeida, Adelaide
中科院分区：
文献类型：
--
作者：
Alves, Eliana;Carvalho, Carla M. B.;Almeida, Adelaide
本文介绍了一种利用重组生物发光细菌监测大肠杆菌光灭活过程的快速简便的方法。将海洋发光细菌Vibriofischeri的luxCDABE基因转化大肠杆菌细胞，并利用重组生物发光指示菌真实的评价了人工（40 W m（-2））和太阳光（约620 W m（-2））照射下3种阳离子meso取代卟啉衍生物对它们代谢活性的影响。研究了生物发光E.大肠杆菌是有效的（> 4 log生物发光降低），使用三种卟啉，三阳离子卟啉Tri-Py+-Me-PF是最有效的化合物。光失活过程是有效的，与太阳光和人工光，为三个卟啉测试。结果表明，生物发光分析是一种有效和灵敏的方法，此外，更负担得起的，更快，更便宜和更少的劳动力比传统的方法。该方法可用作体外细菌光灭活研究的筛选方法，也可用于监测新型光敏剂分子的效率。据我们所知，这是第一个涉及使用生物发光细菌来监测卟啉在环境条件下的抗菌活性的研究。
A faster and simpler method to monitor the photoinactivation process of Escherichia coli involving the use of recombinant bioluminescent bacteria is described here. Escherichia coli cells were transformed with luxCDABE genes from the marine bioluminescent bacterium Vibrio fischeri and the recombinant bioluminescent indicator strain was used to assess, in real time, the effect of three cationic meso-substituted porphyrin derivatives on their metabolic activity, under artificial (40 W m(-2) ) and solar irradiation (approximate to 620 W m(-2)). The photoinactivation of bioluminescent E. coli is effective (> 4 log bioluminescence decrease) with the three porphyrins used, the tricationic porphyrin Tri-Py+-Me-PF being the most efficient compound. The photoinactivation process is efficient both with solar and artificial light, for the three porphyrins tested. The results show that bioluminescence analysis is an efficient and sensitive approach being, in addition, more affordable, faster, cheaper and much less laborious than conventional methods. This approach can be used as a screening method for bacterial photoinactivation studies in vitro and also for the monitoring of the efficiency of novel photosensitizer molecules. As far as we know, this is the first study involving the use of bioluminescent bacteria to monitor the antibacterial activity of porphyrins under environmental conditions.