DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS EYE INFECTION IN TANZANIA BY POLYMERASE CHAIN-REACTION ENZYME-IMMUNOASSAY
DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS EYE INFECTION IN TANZANIA BY POLYMERASE CHAIN-REACTION ENZYME-IMMUNOASSAY
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DOI：
10.1016/0140-6736(91)91502-l
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发表时间：
1991-10-05
期刊：
影响因子：
168.9
通讯作者：
WEST, S
中科院分区：
文献类型：
--
作者：
BOBO, L;MUNOZ, B;WEST, S
沙眼衣原体眼部感染的检测很大程度上是不满意的标准实验室方法。 聚合酶链反应/酶免疫分析（PCR-EIA），以前已成功地诊断生殖器沙眼衣原体感染进行了比较，直接抗体免疫荧光（DFA）检测的有机体在结膜刮伤。 234名1-7岁的坦桑尼亚儿童生活在一个以前没有沙眼控制计划的村庄，临床分类为无沙眼迹象（0）n = 97，滤泡性沙眼（TF）n = 100，或严重炎症性沙眼伴或不伴TF（TI +/- TF）n = 37。 PCR-EIA分别在24%、54%和95%的受试者中检出了沙眼衣原体，而DFA的基本体（EB）检测分别为1%、28%和60%。 沙眼衣原体眼部感染的总患病率为22%的DFA相比，48%的PCR-EIA。 在治疗前有衣原体DNA的受试者中，103例（92%）在4周眼部四环素治疗结束时未检测到衣原体DNA。 结果表明，PCR-EIA可能会影响沙眼诊断和流行病学，因为在所有临床组中检测沙眼衣原体的敏感性增加;对标本采集和运输的要求不太严格，使该方法适合现场使用。 此外，PCR-EIA的半定量方面可能是有用的监测治疗后衣原体DNA的减少。
Detection of Chlamydia trachomatis eye infection is largely unsatisfactory by standard laboratory methods. A polymerase chain reaction/enzyme immunoassay (PCR-EIA) that had previously been successful for diagnosis of genital C trachomatis infection was compared with direct antibody immunofluorescence (DFA) for detection of the organism in conjunctival scrapes. 234 Tanzanian children aged 1-7 years living in a village that had had no previous trachoma control programme were classified clinically as having no sign of trachoma (0) n = 97, follicular trachoma (TF) n = 100, or intense inflammatory trachoma with or without TF (TI +/- TF) n = 37. PCR-EIA detected C trachomatis in 24%, 54%, and 95% of subjects, respectively, compared with elementary body (EB) detection by DFA of 1%, 28%, and 60%, respectively. Overall prevalence of chlamydial eye infection was 22% by DFA compared with 48% by PCR-EIA. Of subjects with chlamydial DNA at pretreatment, 103 (92%) had no detectable chlamydial DNA at the end of 4 weeks of ocular tetracycline. The findings show that PCR-EIA is likely to affect trachoma diagnosis and epidemiology because of the increased sensitivity for detection of C trachomatis in all clinical groups; the less stringent requirements for specimen collection and transport make this method suitable for field use. Moreover, the semi-quantitative aspect of PCR-EIA may be useful for monitoring a decrease in chlamydial DNA after treatment.