Astragalus polysaccharide has a protective effect on hematopoiesis in an irradiated mouse model and decreases apoptosis in megakaryocytes
Astragalus polysaccharide has a protective effect on hematopoiesis in an irradiated mouse model and decreases apoptosis in megakaryocytes
复制标题
黄芪多糖对辐射小鼠模型造血具有保护作用并减少巨核细胞凋亡
DOI：
10.3892/mmr.2020.11653
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发表时间：
2021-01-01
影响因子：
3.4
通讯作者：
Yang, Mo
中科院分区：
文献类型：
--
作者：
Li, Liang;Xu, Wanhua;Yang, Mo
黄芪是黄芪的干燥根，是传统中药中的重要草药，几个世纪以来一直用于促进造血。黄芪多糖（ASPS）是黄芪中的活性成分，在造血过程中起重要作用.本研究的目的是研究造血作用，特别是血小板生成的影响，并使用辐射诱导的骨髓抑制小鼠模型的分子机制的ASPS。采用集落形成单位测定、流式细胞术检测细胞凋亡、ELISA、Giemsa染色和蛋白质印迹法检测ASPS的造血和抗凋亡作用。结果表明，ASPS在治疗后第21天增强了红细胞的恢复，以及在第14天增强了血小板和白色细胞的恢复。此外，ASPS促进所有谱系（巨核细胞、粒细胞单核细胞、红系细胞和成纤维细胞）中的集落形成。骨髓的形态学研究表明，与对照组相比，ASPS和血小板生成素（TPO）治疗组保留了三系造血。ASPS给药组的总体细胞构成（平均总细胞计数/面积）与TPO给药组相似。此外，体外实验表明，用100 μ g/ml ASPS处理对集落形成表现出最大效果。ASPS通过抑制线粒体caspase-3信号通路抑制巨核细胞凋亡。结论：ASPS可能通过促进造血干/祖细胞增殖和抑制巨核细胞凋亡促进辐射骨髓抑制小鼠的造血功能。
Huangqi, the dried root of Radix Astragali, is an essential herb in Traditional Chinese Medicine and has been used to promote hematopoiesis for centuries. Astragalus polysaccharide (ASPS), the bioactive compound of Huangqi, serves a crucial role in hematopoiesis. The aim of the present study was to investigate the hematopoietic effects, in particular the thrombopoietic effects, and the molecular mechanisms of ASPS using an irradiation-induced myelosuppressive mouse model. Colony-forming unit assays, flow cytometric analysis of apoptosis, ELISAs, Giemsa staining and western blotting were performed to determine the hematopoietic and anti-apoptotic effects of ASPS. The results demonstrated that ASPS enhanced the recovery of red blood cells at day 21 following treatment, as well as platelets and white blood cells at day 14. In addition, ASPS promoted colony formation in all lineages (megakaryocytes, granulocyte monocytes, erythroid cells and fibroblasts). The morphological study of the bone marrow demonstrated that tri-lineage hematopoiesis was preserved in the ASPS- and thrombopoietin (TPO)-treated groups compared with the control group. The overall cellularity (mean total cell count/area) of the ASPS-treated group was similar to that of the TPO-treated group. Additionally, in vitro experiments indicated that treatment with 100 mu g/ml ASPS exhibited the maximum effect on colony formation. ASPS attenuated cell apoptosis in megakaryocytic cells via inhibiting the mitochondrial caspase-3 signaling pathway. In conclusion, ASPS promoted hematopoiesis in irradiated myelosuppressive mice possibly via enhancing hematopoietic stem/progenitor cell proliferation and inhibiting megakaryocytes apoptosis.