Impact of pH and application time of meta-phosphoric acid on resin-enamel and resin-dentin bonding
Impact of pH and application time of meta-phosphoric acid on resin-enamel and resin-dentin bonding
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DOI：
10.1016/j.jmbbm.2017.11.028
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发表时间：
2018-02-01
影响因子：
3.9
通讯作者：
Gomes, J. C.
中科院分区：
文献类型：
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作者：
Cardenas, A. F. M.;Siqueira, F. S. F.;Gomes, J. C.
目的：为了评价即刻微剪切树脂-牙釉质粘结强度（pSBS）和即刻和6个月微拉伸粘结强度（mu TBS）以及不同pH值的40%偏磷酸（MPA）和传统的37%正磷酸（OPA）在不同应用时间下的粘结界面的纳米渗漏（NL）。此外，釉质蚀刻图案进行了评价，并通过这些不同组引起的化学/形态学变化进行了evaluated.Materials和方法：198个提取的人磨牙被随机分配到实验组，根据自变量的组合：酸[37%正磷酸（OPA），40%偏磷酸（MPA）在pH值：0.5、1和2]和应用时间[7、15和30 s]。釉质粘结试样的制备和测试在mu SBS。树脂牙本质梁进行了测试，立即或6个月后的水存储在mu TBS测试。使用每个牙齿/时间段的粘结梁评价纳米渗漏。牙釉质蚀刻图案和化学和超微形态学分析也进行了。对mu SBS（MPa）数据进行双因素重复测量ANOVA（酸与应用时间）。对于mu TBS，对添加与施用时间与储存时间的数据进行三因素ANOVA和Tukey检验（α = 0.05）。结果：MPA pH 0.5显示与OPA相似的mu TBS，与釉质（p > 0.05）或牙本质（p > 0.05）上的施用时间无关。OPA提供比MPA更高的纳米渗漏值（p = 0.003）。仅在6个月后观察到OPA的TBS显著降低和NL增加（p = 0.001）。应用时间的增加导致MPA的蚀刻图案更明显。化学分析表明，MPA脱矿牙本质呈现透钙磷石和磷酸八钙峰。MPA暴露的胶原蛋白比OPA少。然而，MPA的最佳结果取决于pH值/应用time.Conclusion：使用40%偏磷酸与pH值为0.5是一种替代酸蚀剂牙本质和牙釉质粘接。此外，由于透钙磷石和磷酸八钙的存在以及减少的脱矿模式，MPA的使用在6个月的时间内保持了树脂-牙本质界面。
Purpose: To evaluate the immediate microshear resin-enamel bond strength (pSBS) and the immediate and 6 month microtensile bond strength (mu TBS) and nanoleakage (NL) of the adhesive interface performed by different pHs of 40% meta-phosphoric acid (MPA) were compared with conventional 37% ortho-phosphoric acid (OPA) under different application times. Additionally, the enamel etching patterns were evaluated and the chemical/ morphological changes induced by these differents groups were evaluated.Materials and methods: One hundred and ninety-eight extracted human molars were randomly assigned into experimental groups according to the combination of independent variables: Acid [37% ortho-phosphoric acid (OPA), 40% meta-phosphoric acid (MPA) at pHs of: 0.5, 1 and 2] and Application Time [7,15 and 30 s]. Enamel bond specimens were prepared and tested under mu SBS. Resin-dentin beams were tested under mu TBS tested immediately or after 6-months of water storage. Nanoleakage was evaluated using bonded-beams of each tooth/time-period. Enamel etching pattern and chemical and ultra-morphology analyses were also performed. The mu SBS (MPa) data were subjected to a two-way repeated measures ANOVA (Acid vs. Application time). For mu TBS, Add vs application time vs storage time data were subjected to three-way ANOVA and Tukey's test (alpha = 0.05).Results: MPA pH 0.5 showed mu TBS similar to OPA, independently of the application time on enamel (p > 0.05) or dentin (p > 0.05). OPA provided higher nanoleakage values than MPA (p = 0.003). Significant decreases in TBS and increases in NL were only observed for OPA after 6 months (p = 0.001). An increase in the application time resulted in a more pronounced etching pattern for MPA. Chemical analysis showed that dentin demineralized by MPA depicted peaks of brushite and octacalcium phosphate. MPA exposed less collagen than OPA. However, optimal results for MPA were dependent on pH/application time.Conclusion: The use of 40% meta-phosphoric acid with a pH of 0.5 is an alternative acid-etching agent for dentin and enamel bonding. Furthermore, the use of MPA preserves the resin-dentin interface over a 6-months period, due to presence of brushite and octacalcium phosphate and a reduced demineralization pattern.