Transcriptome Sequencing and Comparative Analysis of Ovary and Testis Identifies Potential Key Sex-Related Genes and Pathways in Scallop Patinopecten yessoensis
Transcriptome Sequencing and Comparative Analysis of Ovary and Testis Identifies Potential Key Sex-Related Genes and Pathways in Scallop Patinopecten yessoensis
复制标题
卵巢和睾丸的转录组测序和比较分析鉴定了扇贝扇贝性别相关的潜在关键基因和通路
DOI：
10.1007/s10126-016-9706-8
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发表时间：
2016-08-01
影响因子：
3
通讯作者：
Bao, Zhenmin
中科院分区：
文献类型：
--
作者：
Li, Yangping;Zhang, Lingling;Bao, Zhenmin
双壳类软体动物有着令人着迷的多种繁殖方式。然而，对这类动物的性别决定和生殖调节的研究仍处于起步阶段。本研究对叶索扇贝的三个卵巢和三个睾丸的转录组进行了测序和分析。转录组比较显示，4394个基因在卵巢和睾丸之间存在显著差异，其中1973个基因为卵巢偏倚（在卵巢中上调），2421个基因为睾丸偏倚。先前在脊椎动物中报道并推测存在于双壳类动物中的关键性别决定基因，即FOXL2， DMRT， SOXH和SOXE，进行了研究。这些基因都具有保守的功能域，并在性腺中检测到。除pysoxe外，其余3个基因在卵巢和睾丸间表达均有显著差异。pyfoxl22是卵巢偏倚的，pydmrtandpysoxh是睾丸偏倚的，这表明这三个基因可能是扇贝性别决定/分化的关键候选基因。此外，对卵巢和睾丸偏倚基因进行了GO和KEGG富集分析。有趣的是，神经递质转运体和GABA能突触基因在卵巢偏倚基因中都被过度代表，这表明GABA和甘氨酸等神经递质可能参与了扇贝卵巢的发育。我们的研究将有助于更好地理解双壳类动物性别决定和生殖调节的分子机制。
Bivalve mollusks have fascinatingly diverse modes of reproduction. However, research investigating sex determination and reproductive regulation in this group of animals is still in its infancy. In this study, transcriptomes of three ovaries and three testes of Yesso scallop were sequenced and analyzed. Transcriptome comparison revealed that 4394 genes were significantly different between ovaries and testes, of which 1973 were ovary-biased (upregulated in the ovaries) and 2421 were testis-biased. Crucial sex-determining genes that were previously reported in vertebrates and putatively present in bivalves, namely FOXL2, DMRT, SOXH, and SOXE, were investigated. The genes all possessed conserved functional domains and were detected in the gonads. Except forPySOXE, the other three genes were significantly differentially expressed between the ovaries and testes.PyFOXL2was ovary-biased, andPyDMRTandPySOXHwere testis-biased, suggesting that these three genes are likely to be key candidates for scallop sex determination/differentiation. Furthermore, GO and KEGG enrichment analyses were conducted for both ovary- and testis-biased genes. Interestingly, both neurotransmitter transporters and GABAergic synapse genes were overrepresented in the ovary-biased genes, suggesting that neurotransmitters, such as GABA and glycine, are likely to participate in scallop ovary development. Our study will assist in better understanding of the molecular mechanisms underlying bivalve sex determination and reproductive regulation.