Estrogen-induced alterations of spinal cord enkephalin gene expression
Estrogen-induced alterations of spinal cord enkephalin gene expression
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DOI：
10.1016/s0304-3959(99)00109-8
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发表时间：
1999-11-01
期刊：
影响因子：
7.4
通讯作者：
Blomqvist, A
中科院分区：
文献类型：
--
作者：
Amandusson, Å;Hallbeck, M;Blomqvist, A
脊髓和三叉背角浅层的脑啡肽合成神经元是内源性疼痛调节系统的重要组成部分。我们之前已经证明这些神经元显示细胞内雌激素受体，这表明雌激素可能影响它们的脑啡肽表达。通过Northern blot，我们现在发现，在去卵巢的雌性大鼠中，大量注射雌激素导致脊髓脑啡肽mRNA水平迅速增加。由此可见，雌激素给药4 h后，大鼠腰椎脑啡肽mrna的表达量比只注射载药的对照组平均高68% （P < 0.05）。8小时后，脑啡肽mRNA的数量也有小幅增加（P < 0.05），而注射雌激素的动物和对照动物在24小时后或短于4小时的时间内没有发现差异。结合先前的解剖学数据，本研究结果表明，雌激素对涉及伤害性信息传递区域的脊髓阿片水平有急性影响。(C) 1999年国际疼痛研究协会。Elsevier Science B.V.出版
Enkephalin-synthesizing neurons in the superficial laminae of the spinal and trigeminal dorsal horn are critical components of the endogenous pain-modulatory system. We have previously demonstrated that these neurons display intracellular estrogen receptors, suggesting that estrogen can potentially influence their enkephalin expression. By using Northern blot, we now show that a bolus injection of estrogen results in a rapid increase in spinal cord enkephalin mRNA levels in ovariectomized female rats. Thus, 4 h after estrogen administration the enkephalin mRNA-expression in the lumbar spinal cord was on average 68% higher (P < 0.05) than in control animals injected with vehicle only. A small increase in the amount of enkephalin mRNA was also seen after 8 h (P < 0.05), whereas no difference between estrogen-injected and control animals was found after 24 h or at time periods shorter than 4 h. Taken together with the previous anatomical data, the present findings imply that estrogen has an acute effect on spinal opioid levels in areas involved in the transmission of nociceptive information. (C) 1999 International Association for the Study of Pain. Published by Elsevier Science B.V.