Nondestructive evaluation of egg-to-adult development and feeding behavior of the bamboo powderpost beetle Dinoderus minutus using X-ray computed tomography
Nondestructive evaluation of egg-to-adult development and feeding behavior of the bamboo powderpost beetle Dinoderus minutus using X-ray computed tomography
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利用 X 射线计算机断层扫描无损评估竹粉甲虫 Dinoderus minutus 的卵到成虫的发育和摄食行为
DOI：
10.1007/s10086-017-1642-3
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发表时间：
2017
影响因子：
2.9
通讯作者：
Fujii Yoshihisa
中科院分区：
文献类型：
--
作者：
Watanabe Hiroki;Yanase Yoshiyuki;Fujii Yoshihisa
由于竹粉虫的产卵、发育和取食都发生在竹秆内，因此对竹粉虫的生活史和取食生物学研究还很有限。在这项研究中，X射线计算机断层扫描（CT）的非破坏性量化的进展，幼虫的身体大小和隧道大小从第一龄化蛹。鸡蛋D。容易收集夹在含营养物的滤纸层压物之间的微粒。将初孵幼虫单独接种于毛竹的节间。每3-5天使用微焦点X射线CT系统扫描这些碎片，以使甲虫的身体和隧道可视化，分辨率为18-60 μm/体素。成虫羽化后继续进行CT扫描，以分析交配前成虫的进食情况。卵长0.84 ± 0.06mm，卵期5.0 ± 0.8d。根据CT图像，幼虫生长至体长3.53 ± 0.23 mm，变成3.42 ± 0.09 mm的脓。幼虫期为61 ± 11天，钻洞长度为80.2 ± 4.8 mm，体积为68.0 ± 7.0 mm 3。成虫羽化后8 ± 1d内，成虫在竹片上取食，然后钻出出洞。在此期间，他们钻隧道的速度为2.64 ± 0.58毫米/天的长度和4.87 ± 1.10毫米3/天的体积。
The life history and feeding biology of the bamboo powderpost beetleDinoderus minutusremain poorly understood because the beetles’ oviposition, development, and feeding take place inside bamboo culms. In this study, X-ray computed tomography (CT) was employed to nondestructively quantify the progression of larval body size and tunnel size from the first instar to pupation. Eggs ofD. minutuslaid between laminates of nutrient-containing filter paper were easily collected. The newly hatched larvae were inoculated singly into pieces of internodes of madake (Phyllostachys bambusoides). The pieces were scanned using a microfocus X-ray CT system every 3–5 days to visualize the beetles’ bodies and tunnels with resolutions of 18–60 μm/voxel. CT scans were continued after adult eclosion to analyze pre-mating adult feeding. The collected eggs were 0.84 ± 0.06 mm (mean ± SD) in length and the egg duration lasted 5.0 ± 0.8 days. Based on CT images, the larvae grew to 3.53 ± 0.23 mm in body length and turned into pupae of 3.42 ± 0.09 mm. The larvae bored tunnels with a length of 80.2 ± 4.8 mm and a volume of 68.0 ± 7.0 mm3over the larval period of 61 ± 11 days. Newly emerged adults remained in the bamboo pieces to feed before making exit holes in 8 ± 1 days after adult eclosion. During this period, they bored tunnels at rates of 2.64 ± 0.58 mm/day in length and 4.87 ± 1.10 mm3/day in volume.
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