Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A
Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A
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岛硫化叶菌 REY15A 两个 DExD/H-box 家族解旋酶基因的敲除及功能分析
DOI：
10.1007/s00792-016-0847-5
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发表时间：
2016-06
期刊：
影响因子：
2.9
通讯作者：
Shen Yulong
中科院分区：
文献类型：
--
作者：
Song Xueguo;Huang Qihong;Ni Jinfeng;Yu Yang;Shen Yulong
DExD/H - 盒解旋酶是最大的解旋酶家族。它们属于第二超家族解旋酶，参与核苷酸代谢、核糖体生物发生以及核质运输。一些DExD/H的生化特性和结构……
DExD/H-box helicases represent the largest family of helicases. They belong to superfamily 2 helicases and participate in nucleotide metabolism, ribosome biogenesis, and nucleocytoplasmic transport. The biochemical properties and structures of some DExD/H-box helicases in the archaea have been documented, but many of them have not been characterized; and reports on in vivo functional analyses are limited. In this study, we attempted gene knockout of 8 putative DExD/H-box helicases in Sulfolobus islandicus REY15A and obtained two deletion mutants, SiRe_0681 and SiRe_1605. We determined that ΔSiRe_0681 grew faster than wild type cells in the presence of methyl methanesulfonate (MMS). Flow cytometry analysis showed that this strain had fewer G1/S phase cells than the wild type, and the genes coding for cell division proteins were up-regulated. The stain ΔSiRe_1605 was more sensitive to MMS than the wild type cell, and many nucleotide metabolism and DNA repair enzymes were found to be down-regulated. Intriguingly, deletion of either gene led to silencing simultaneously of over 80 genes located at a specific region. This study provides a novel insight into the in vivo functions of predicted DExD/H-box family helicases in the archaea.
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影响因子：
3.8
作者：
Hong, Ye;Chu, Mingzhu;Shen, Yulong
通讯作者：
Shen, Yulong
DOI：
10.1016/j.bbrc.2009.09.038
发表时间：
2009-11
影响因子：
3.1
作者：
Y. Shimada;Wakao Fukuda;Yohei Akada;Mayumi Ishida;J. Nakayama;T. Imanaka;S. Fujiwara
通讯作者：
Y. Shimada;Wakao Fukuda;Yohei Akada;Mayumi Ishida;J. Nakayama;T. Imanaka;S. Fujiwara
影响因子：
64.5
作者：
Boshoff, HIM;Reed, MB;Mizrahi, V
通讯作者：
Mizrahi, V
影响因子：
4.8
作者：
Fujikane, R;Komori, K;Ishino, Y
通讯作者：
Ishino, Y
影响因子：
5.6
作者：
Isabel L Woodman;G. S. Briggs;E. Bolt
通讯作者：
Isabel L Woodman;G. S. Briggs;E. Bolt