Leaf epidermal characters of Lonicera japonica and Lonicera confuse and their ecology adaptation
Leaf epidermal characters of Lonicera japonica and Lonicera confuse and their ecology adaptation
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DOI：
10.1007/s11676-007-0020-1
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发表时间：
2007-06
影响因子：
3
通讯作者：
Qiang Li;Longjiang Yu;Yan Deng;Wei Li;Maoyuan Li;Jian-hua Cao
中科院分区：
文献类型：
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作者：
Qiang Li;Longjiang Yu;Yan Deng;Wei Li;Maoyuan Li;Jian-hua Cao
本文报道了日本忍冬（EscherajaponicaThunb.）利用扫描电镜对山银花属植物山银花和野生山银花的气孔、表皮毛、表皮细胞等特征进行了观察。结果表明，气孔仅分布于下表皮，分布不规则，叶表皮由表皮细胞、气孔复合体和浓密的腺毛和非腺毛组成。上表皮垂周壁凹陷成波状沟状，阻碍蒸腾作用。叶片形态结构的变化为植物抗旱机理提供了又一证据。在Fluo-3/AM激光扫描共聚焦显微镜下观察到一些规则分布的瘤状突起，推测其为草酸钙，可增强其对干旱胁迫的耐受性。因此，山银花的上述特性可以减少水分的流失，使金银花和野蜜适应西南喀斯特地区的干旱环境。然而，这两个物种之间存在一些差异。扫描电子显微镜观察结果表明，金银花上表皮有规则的腺毛沿着中脉分布，而野蜜上表皮无腺毛，这一结果可验证野蜜种间的亲缘关系，并为野蜜的分类提供一定的意义。
The leaf epidermis of Japanese honeysuckle (Lonicera japonicaThunb.) and Wild Honeysuckle (Lonicera confusa) in the genus ofFlos Loniceraewere mainly observed by scanning electron microscopes (SEM) to study the characteristics of stomata, trichomes and dermal cell,etc.. The results showed that stoma exists only on the lower epidermis and its distribution is irregular, and leaf epidermis consist of epidermis cells, stoma complexes and bushy trichomes including glandular hair and non-glandular hair. On the upper epidermis, anticlinal wall caves in sinuous groove to countercheck the transpiration. Evidences from leaf morphological structures serve as another proof on drought-resistant mechanisms. Some strumaes distributing regularly are hypothesized as oxalic calcium on the lower epidermis under laser scanning confocal microscopy (LSCM) with Fluo-3/AM, which can increase their endurance to drought stress. Therefore, the above characteristics ofFlos Loniceraecan reduce the loss of water and make Japanese honeysuckle and Wild Honeysuckle adapt to the droughty environment at Karst area in southwest China. However, there is some difference of the two species. From the SEM (Scanning Electron Microscopy) result, it is shown that on the upper epidermis, some glandular hair regularly present along the midrib of Japanese honeysuckle, but Wild Honeysuckle has no glandular hair on the upper epidermis, which can verify the relationships ofFlos Loniceraespecies and provide the significance for classification ofFlos Lonicerae.