Whole genome deep sequencing revealed host impact on population structure, variation and evolution of Rice stripe virus
Whole genome deep sequencing revealed host impact on population structure, variation and evolution of Rice stripe virus
复制标题
全基因组深度测序揭示宿主对水稻条纹病毒群体结构、变异和进化的影响
DOI：
10.1016/j.virol.2018.08.005
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发表时间：
2018-11-01
期刊：
影响因子：
3.7
通讯作者：
Wu, Jianxiang
中科院分区：
文献类型：
--
作者：
Lu, Lina;Wu, Sanling;Wu, Jianxiang
High-throughput deep sequencing and variant detection showed that variations of Rice stripe virus (RSV) populations obtained from small brown planthopper-transmitted rice plants and sap-inoculated N. benthamiana plants were single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels). The SNPs were more uniform across RSV genome, but InDels occurred mainly in the intergenic regions (IRs) and in the 5' or 3' noncoding regions. There were no clear patterns of InDels, although the inserted sequences were all from virus itself. Six, one, and one non-synonymous substitutions were respectively observed in the RdRP ORF, IR and the movement protein ORF. These non-synonymous substitutions were found to be stable, resulting in new consensus sequences in the NBL11 RSV population. Furthermore, the numbers of SNPs and InDels in RSV genome from N. benthamiana plants were much higher than that from O. sativa plants. These differences are likely caused by selection pressures generated by different host plants.
