The promise of single-cell sequencing
The promise of single-cell sequencing
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DOI：
10.1038/nmeth.2769
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发表时间：
2014-01-01
期刊：
影响因子：
48
通讯作者：
Kim, Junhyong
中科院分区：
文献类型：
--
作者：
Eberwine, James;Sul, Jai-Yoon;Kim, Junhyong
具有相同表型的单个细胞通常被视为组织或器官的相同功能单位。然而，来自单细胞的DNA和RNA的深度测序表明，异质细胞状态的更复杂的生态共同产生紧急的系统级功能。高含量、实时、多模态单细胞测量技术的持续发展将导致理解单个细胞在其微环境中的功能的最终目标。
Individual cells of the same phenotype are commonly viewed as identical functional units of a tissue or organ. However, the deep sequencing of DNA and RNA from single cells suggests a more complex ecology of heterogeneous cell states that together produce emergent system-level function. Continuing development of high-content, real-time, multimodal single-cell measurement technologies will lead to the ultimate goal of understanding the function of an individual cell in the context of its microenvironment.