Expression of cytokeratin 7 and 20 in pathological conditions of the bile tract
Expression of cytokeratin 7 and 20 in pathological conditions of the bile tract
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DOI：
10.1078/0344-0338-00356
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Aragona, F
中科院分区：
文献类型：
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作者：
Cabibi, D;Licata, A;Aragona, F
细胞角蛋白 7 (CK7) 和细胞角蛋白 20 (CK20) 的表达有助于确定胆道癌和转移性癌的起源。我们研究了 CK7 和 CK20 在胆管炎症、化生和肿瘤性疾病中的表达，并评估了 CK 表达模式与肝外胆管/胆囊癌 (EBDC) 或肝内胆管癌 (IBDC) 之间的可能关系。我们使用免疫组织化学方法研究了 48 个福尔马林固定、石蜡包埋的标本，分为：A) 石质或手术切除发炎的肝外胆管/胆囊：均为CK7(+)/CK20(+)； B)慢性丙型肝炎原发性胆汁性肝硬化和原发性硬化性胆管炎患者经皮肝活检：均为CK7(+)/CK20(-)； C) EBDC：除两例为 CK7(-)/CK20(-) 外，均为 CK7(+)/CK20(+)； D) IBDC：除 1 例 CK20 阳性外，均为 CK7(+)/CK20(-)。仅在A组和C组的标本中观察到化生改变：在这些情况下，CK20要么局灶性表达，要么弥漫性表达。我们的研究表明，特定刺激下细胞角蛋白的表达可能与正常组织不同，有时CK20表达可能与化生改变的发生相关或先于化生改变的发生。
Expression of cytokeratin 7 (CK7) and cytokeratin 20 (CK20) helps to establish the origin of biliary and metastatic carcinomas. We investigated the expression of CK7 and CK20 in inflammatory, metaplastic and neoplastic conditions of the bile ducts, and evaluated possible relationships between the CK expression pattern and extrahepatic bile duct/gallbladder carcinomas (EBDCs) or intrahepatic bile duct carcinomas (IBDCs).We used immunohistochemistry for the investigation of 48 formalin-fixed, paraffin-embedded specimens grouped as: A) lithiasic or inflamed surgically resected extrahepatic bile ducts/gallbladders: all were CK7(+)/CK20(+); B) percutaneous liver biopsies from patients with chronic hepatitis C primary biliary cirrhosis and primary sclerosing cholangitis: all were CK7(+)/CK20(-); C) EBDCs: all were CK7(+)/CK20(+), except for two cases which were CK7(-)/CK20(-); D) IBDCs: all were CK7(+)/CK20(-), except for one case showing CK20 positivity. Metaplastic changes were seen only among specimens in groups A and C: in these cases, CK20 was either focally or diffusely expressed.Our study suggests that the expression of cytokeratins under specific stimuli can be different from normal tissues, and that sometimes CK20 expression can be related to and precede the occurrence of metaplastic alterations.