Hsa_circ_0054633 in peripheral blood can be used as a diagnostic biomarker of pre-diabetes and type 2 diabetes mellitus.
Hsa_circ_0054633 in peripheral blood can be used as a diagnostic biomarker of pre-diabetes and type 2 diabetes mellitus.
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外周血中的Hsa_circ_0054633可作为糖尿病前期和2型糖尿病的诊断生物标志物。
DOI：
10.1007/s00592-016-0943-0
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发表时间：
2017-03
影响因子：
3.8
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Zhao Z;Li X;Jian D;Hao P;Rao L;Li M
本研究的目的是研究环状rna （circRNAs）在2型糖尿病（T2DM）患者外周血中的特征表达及其作为糖尿病前期和T2DM诊断生物标志物的潜力。我们收集了6名健康个体和6名T2DM患者外周血中的circrna进行微阵列分析，并对20名正常病例、20名糖尿病前期患者和20名T2DM患者进行了独立队列研究，以验证所选择的5种circrna。然后，我们在第三个队列（对照组，n = 60；糖尿病前期组，n = 63； T2DM组，n = 64）中通过定量实时聚合酶链反应（Q-PCR）检测hsa_circ_0054633。总共发现489个circrna在两组之间存在差异表达，其中T2DM组中78个表达上调，411个表达下调。然后选择5个circrna作为候选生物标志物，并在第二个队列中进一步验证。Hsa_circ_0054633的曲线下面积（AUC）最大。在第三个队列中测试了hsa_circ_0054633的诊断能力。引入T2DM危险因素后，诊断糖尿病前期和T2DM的hsa_circ_0054633 auc分别从0.751（95%可信区间[0.666-0.835],P < 0.001）上升至0.841 ([0.773-0.910],P < 0.001)，从0.793 （[0.716-0.871],P < 0.001）上升至0.834 （[0.762-0.905],P < 0.001）。Hsa_circ_0054633对糖尿病前期和T2DM具有一定的诊断能力。本文的在线版本（doi:10.1007/s00592-016-0943-0）包含补充材料，可供授权用户使用。
The purpose of the current study was to investigate the characteristic expression of circular RNAs (circRNAs) in the peripheral blood of type 2 diabetes mellitus (T2DM) patients and their potential as diagnostic biomarkers for pre-diabetes and T2DM. CircRNAs in the peripheral blood from six healthy individuals and six T2DM patients were collected for microarray analysis, and an independent cohort study consisting of 20 normal cases, 20 pre-diabetes patients and 20 T2DM patients was conducted to verify the five chosen circRNAs. We then tested hsa_circ_0054633 in a third cohort (control group, n = 60; pre-diabetes group, n = 63; and T2DM group, n = 64) by quantitative real-time polymerase chain reaction (Q-PCR). In total, 489 circRNAs were discovered to be differentially expressed between the two groups, and of these, 78 were upregulated and 411 were downregulated in the T2DM group. Five circRNAs were then selected as candidate biomarkers and further verified in a second cohort. Hsa_circ_0054633 was found to have the largest area under the curve (AUC). The diagnostic capacity of hsa_circ_0054633 was tested in a third cohort. After introducing the risk factors of T2DM, the hsa_circ_0054633 AUCs for the diagnosis of pre-diabetes and T2DM slightly increased from 0.751 (95% confidence interval [0.666–0.835], P < 0.001) to 0.841 ([0.773–0.910], P < 0.001) and from 0.793 ([0.716–0.871], P < 0.001) to 0.834 ([0.762–0.905], P < 0.001), respectively. Hsa_circ_0054633 presented a certain diagnostic capability for pre-diabetes and T2DM. The online version of this article (doi:10.1007/s00592-016-0943-0) contains supplementary material, which is available to authorized users.