Inactivation of MED-1 elements in the TATA-less, initiator-less mouse thymidylate synthase promoter has no effect on promoter strength or the complex pattern of transcriptional start sites.
Inactivation of MED-1 elements in the TATA-less, initiator-less mouse thymidylate synthase promoter has no effect on promoter strength or the complex pattern of transcriptional start sites.
复制标题
无 TATA、无起始子的小鼠胸苷酸合酶启动子中 MED-1 元件的失活对启动子强度或转录起始位点的复杂模式没有影响。
DOI：
10.1002/(sici)1097-4644(19990401)73:1
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发表时间：
1999
影响因子：
4
通讯作者：
Johnson,LF
中科院分区：
文献类型：
--
作者：
Rudge,TL;Johnson,LF
The mouse thymidylate synthase (TS) promoter is a member of a family of promoters that lack a TATA box as well as an initiator element and that initiate transcription at many sites over a broad initiation window. An element (MED‐1) downstream of the initiation window of almost all promoters of this family has been proposed to be important for promoter activity, as well as for multiple start site utilization. Two consensus MED‐1 elements are located downstream of the initiation window of the TS promoter. To determine the role of the MED‐1 elements in the TS promoter, one or both elements were inactivated by site‐directed mutagenesis and the effects on promoter function were determined. We found that inactivation of the MED‐1 elements had no measurable effect on promoter strength, the boundaries of the initiation window, or the pattern of transcriptional start sites. Furthermore, inactivation of the elements did not affect the ability of the TS promoter to direct S phase‐specific expression of the gene in growth‐stimulated cells. We conclude that the MED‐1 element does not play a significant role in TS promoter function and therefore is not an essential component of all TATA‐less promoters with complex transcriptional initiation patterns. J. Cell. Biochem. 73:90–96, 1999. © 1999 Wiley‐Liss, Inc.
