Insulin injections promote the growth of aberrant crypt foci in the colon of rats
Insulin injections promote the growth of aberrant crypt foci in the colon of rats
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DOI：
10.1080/01635589709514543
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发表时间：
1997-01-01
影响因子：
2.9
通讯作者：
Tache, S
中科院分区：
文献类型：
--
作者：
Corpet, DE;Jacquinet, C;Tache, S
本研究的主要目的是验证外源性胰岛素会促进结肠癌发生的假设。36只雌性Fischer 344大鼠自由采食低脂啮齿类动物饲料，并单次注射氧化偶氮甲烷（20 mg/kg）;一周后，将其随机分为两组。对照组大鼠皮下注射生理盐水，5天/周，实验组大鼠给予超长效牛胰岛素（20 U/kg）。通过治疗100天（72次注射）后异常隐窝病灶的多样性（隐窝数量）评估胰岛素注射的促进作用。给予胰岛素的大鼠比对照组吃得更多，体重也更重（215 +/- 11 vs. 182 +/- 7 g，p < 0.001）。胰岛素注射还增加了腹部脂肪、血浆甘油三酯和胰岛素血症的量，并降低了血糖（所有p < 0.05）。两组中异常隐窝病灶的数量相同，但胰岛素注射显着增加了其多样性（对照组为2.8 +/- 0.3 vs 2.5 +/- 0.2个隐窝/病灶，p = 0.007）。此外，注射胰岛素的大鼠中唾液粘蛋白产生灶的比例高于对照组（p = 0.04）。这些数据表明，外源性胰岛素可以促进大鼠结肠癌的发生，并表明导致低血胰岛素的生活方式和饮食可能保护人类免受结直肠癌的侵害。
The main objective of the present study was to test the hypothesis that exogenous insulin would enhance colon carcinogenesis. Thirty-six female Fischer 344 rats were fed ad libitum a low-fat rodent chow and given a single azoxymethane injection (20 mg/kg); one week later, they were randomized into two groups. Control rats were given a subcutaneous saline injection, 5 days/wk, and experimental rats were given Ultralente bovine insulin (20 U/kg). The promoting effect of insulin injections was assessed by the multiplicity (number of crypts) of aberrant crypt foci after 100 days of treatment (72 injections). The rats given insulin ate more and were heavier than controls (215 +/- 11 vs. 182 +/- 7 g, p < 0.001). Insulin injections also increased the amount of abdominal fat, plasma triglycerides, and insulinemia and decreased blood glucose (all p < 0.05). The number of aberrant crypt foci was the same in both groups, bur their multiplicity was significantly increased by the insulin injections (2.8 +/- 0.3 vs. 2.5 +/- 0.2 crypt/focus in controls, p = 0.007). In addition, the proportion of sialomucin-producing foci was higher in insulin-injected rats than in controls (p = 0.04). These data show that exogenous insulin can promote colon carcinogenesis in rats and suggest that life-style and diets leading to low blood insulin might protect humans against colorectal cancer.