PREVALENCE OF F107 FIMBRIAE ON ESCHERICHIA-COLI ISOLATED FROM PIGS WITH EDEMA DISEASE OR POSTWEANING DIARRHEA
PREVALENCE OF F107 FIMBRIAE ON ESCHERICHIA-COLI ISOLATED FROM PIGS WITH EDEMA DISEASE OR POSTWEANING DIARRHEA
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DOI：
10.1016/0378-1135(94)90111-2
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发表时间：
1994-06-01
影响因子：
3.3
通讯作者：
LINTERMANS, P
中科院分区：
文献类型：
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作者：
IMBERECHTS, H;BERTSCHINGER, HU;LINTERMANS, P
该研究包括50头4至12周龄的猪，这些猪死于水肿病或严重腹泻。取十二指肠、空肠和回肠粘膜涂片，用免疫荧光法检测F107菌毛抗原。E.从肠中分离大肠杆菌菌株，并通过玻片凝集（血清群和F107菌毛产生）、通过它们对Vero细胞的细胞毒性和通过基因扩增（编码主要F107亚基FedA、引起水肿疾病的毒素SLT-IIv和肠毒素LTI、STIa和STII的基因）来表征。F107菌毛与E.大肠杆菌O 139：K12和O 141：K85 a，B，而O 149：K91：F4 a，c.一些分离株在F107菌毛培养物中的表达为ETEC菌株产生这些菌毛提供了额外的证据。在O 149：K91：F4 a，c血清群中未检测到SLT-IIv的遗传决定子。fedA基因在两个分离株中被证明是缺乏SLT-IIv。水肿病病例的大多数分离株属于血清群O 139：K12，不含肠毒素基因。来自腹泻猪的分离株血清分型为O 141：K85 a，B或O 149：K91：F4 a，c，基因组中至少携带两种肠毒素基因。在一小部分的情况下，F107抗原显示在肠道涂片中，虽然基因fedA没有检测到相应的分离株。结果证实了F107菌毛作为水肿病E.大肠杆菌菌株，但也表明F107菌毛可以发现与断奶后腹泻分离株。在这些后者菌株肠毒素总是被证明，无论毒素SLT-IIv的存在。
The study comprises fifty 4 to 12 weeks old pigs that died from oedema disease or severe diarrhoea. Smears were prepared from the mucosa of duodenum, jejunum and ileum, and by immunofluorescence F107 fimbrial antigens were detected. E. coli strains were isolated from the intestines and were characterised by slide agglutination (serogroup and F107 fimbriae production), by their cytotoxicity for Vero cells, and by gene amplification (genes coding for the major F107 subunit FedA, the toxin causing oedema disease SLT-IIv, and enterotoxins LTI, STIa and STII). F107 fimbriae were demonstrated in association with E. coli of serogroups O139:K12 and O141:K85a,b but not of serogroup O149:K91 :F4a,c. Expression in culture of F107 fimbriae by some isolates gave additional evidence for production of these fimbriae by ETEC strains. The genetic determinant of SLT-IIv was found in association with F107, and could not be detected in serogroup O149:K91:F4a,c. Gene fedA was demonstrated in two isolates which were devoid of SLT-IIv. Most isolates from cases of oedema disease belonged to serogroup O139:K12 and did not contain enterotoxin genes. Isolates from pigs that suffered from diarrhoea were serotyped O141:K85a,b or O149:K91:F4a,c, and carried at least two enterotoxin genes in their genomes. In a small proportion of the cases F107 antigens were demonstrated in intestinal smears although gene fedA was not detected in the corresponding isolates. The results confirm the importance of F107 fimbriae as virulence factor in oedema disease E. coli strains, but also demonstrate that F107 fimbriae can be found in association with postweaning diarrhoea isolates. In these latter strains enterotoxins were always demonstrated, irrespective of the presence of toxin SLT-IIv.