Polymorphism of Heart Fatty Acid-Binding Protein Gene Associatied with Fatness Traits in the Chicken
Polymorphism of Heart Fatty Acid-Binding Protein Gene Associatied with Fatness Traits in the Chicken
复制标题
DOI：
10.1080/10495390601038900
复制
发表时间：
2007-04
影响因子：
3.7
通讯作者：
Qigui Wang;Hui Li;Ľ. Leng;Yu-xiang Wang;Z. Tang;Ning Li;Fuchun Zhang
中科院分区：
文献类型：
--
作者：
Qigui Wang;Hui Li;Ľ. Leng;Yu-xiang Wang;Z. Tang;Ning Li;Fuchun Zhang
脂肪酸结合蛋白（Fatty Acid-binding Proteins，FABP）是一类对长链脂肪酸具有高亲和力的脂类结合蛋白，在脂类代谢和细胞内转运中发挥重要作用。本研究旨在研究心脏（H）-FABP基因对鸡生长和体组成性状的影响。本研究以东北农业大学肉鸡腹脂分离系和肉×丝羽乌骨鸡F2代为研究对象。测定了群体的体重和体成分性状。根据鸡H-FABP基因序列设计引物。DNA测序检测亲本间的多态性。采用PCR-RFLP和PCR-fragment length polymorphism两种方法对这些群体进行基因分型。结果表明，H-FABP基因多态性与腹脂率相关。表明H-FABP基因可能是影响鸡腹脂含量的候选基因或与主效基因连锁。
Fatty acid-binding proteins (FABP) belong to a superfamily of lipid binding proteins that exhibit a high affinity for long chain fatty acids and appear to function in metabolism and intracellular transportation of lipids. The current study was designed to investigate the effects of heart (H)-FABP gene on chicken growth and body composition traits. The Northeast Agricultural University divergent broiler lines for abdominal fat and a broiler X silkie F2 population were used in this study. Body weight and body composition traits were measured in the populations. Primers were designed according to the chicken H-FABP gene sequence. Polymorphisms between parental lines were detected by DNA sequencing. PCR-RFLP and PCR-fragment length polymorphism methods were developed to genotype the populations. The results showed that the H-FABP gene polymorphisms in the two populations were associated with abdominal fat percentage. It implied that H-FABP gene can be a candidate locus or linked to a major gene(s) that affects abdominal fat content in the chicken.