Fusarium fujikuroi Species Complex Associated With Rice, Maize, and Soybean From Jiangsu Province, China: Phylogenetic, Pathogenic, and Toxigenic Analysis
Fusarium fujikuroi Species Complex Associated With Rice, Maize, and Soybean From Jiangsu Province, China: Phylogenetic, Pathogenic, and Toxigenic Analysis
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与中国江苏省水稻、玉米和大豆相关的 Fujikuroi 镰孢菌复合体：系统发育、致病性和产毒分析
DOI：
10.1094/pdis-09-19-1909-re
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发表时间：
2020-08-01
期刊：
影响因子：
4.5
通讯作者：
Shi, Jianrong
中科院分区：
文献类型：
--
作者：
Qiu, Jianbo;Lu, Yunan;Shi, Jianrong
Fusarium fujikuroi Fusarium Fusarium fujikuroi Fusarium Species complex （FFSC）属物种至关重要，是世界范围内食品数量和质量的主要问题，因为它们不仅导致作物严重疾病，而且还产生各种真菌毒素。为了鉴定该菌株的种群结构和毒性次生代谢物的风险，从江苏省的水稻、玉米和大豆样品中分离出237株候选菌株。通过对翻译延伸因子la基因（TEF-1 α）和伏马菌素（FB）合成基因（FUM1）的测序完成菌株的种类鉴定。观察了镰刀菌在不同作物中的分布情况。玉米种子中以增繁镰刀菌（远形菌，中间赤霉菌）和黄萎病镰刀菌（远形菌，念珠菌）为主，而水稻和大豆样品中分离频率最高的是藤黑镰刀菌（远形菌，藤黑镰刀菌）。此外，对这些菌株进行了系统发育分析，结果显示出与源无明显关系的明确类群。研究了FFSC种的致病性和毒力。所有物种都降低了水稻种子发芽率，但差异不显著。fujikuroi对水稻幼苗长度的影响表现出两种不同的模式，这两种模式与FBs和赤霉素合成有关。FBs主要由黄萎病假单胞菌和增殖假单胞菌产生。增殖F.和fujikuroi F.也产生念珠双胍和beauvericin。F. and diyazi （teleomorph， G. and diyazi）的毒力极低。进一步分析表明，TEF-1 α序列的差异和毒素合成基因表达水平的差异与菌株次生代谢物的多样性有关。这些发现有助于深入了解FFSC的人群水平特征，并可能有助于制定疾病和真菌毒素管理策略。
Species belonging to the Fusarium fujikuroi species complex (FFSC) are of vital importance and are a major concern for food quantity and quality worldwide, as they not only cause serious diseases in crops but also produce various mycotoxins. To characterize the population structure and evaluate the risk of poisonous secondary metabolites, a total of 237 candidate strains were isolated from rice, maize, and soybean samples in Jiangsu Province, China. Species identification of the individual strain was accomplished by sequencing the translation elongation factor la gene (TEF-1 alpha) and the fumonisin (FB) synthetic gene (FUM1). The distribution of Fusarium species among the different crops was observed. The maize seeds were dominated by F. proliferatum (teleomorph, Gibberella intermedia) and F. verticillioides (teleomorph, G. moniliformis), whereas F. fujikuroi (teleomorph, G. fujikuroi) was the most frequently isolated species from rice and soybean samples. In addition, phylogenetic analyses of these strains were performed, and the results suggested clear groups showing no obvious relationship with the origin source. FFSC species pathogenicity and toxigenicity were studied. All of the species reduced the rice seed germination rate, with no significant differences. F. fujikuroi showed two distinct patterns of influencing the length of rice seedlings, which were correlated with FBs and gibberellic acid synthesis. FBs were mainly produced by F. verticillioides and F. proliferatum. F. proliferatum and F. fujikuroi also produced moniliformin and beauvericin. The toxigenicity of F. andiyazi (teleomorph, G. andiyazi) was extremely low. Further analysis indicated that the sequence variations in TEF-1 alpha and the differences in the expression levels of the toxin synthesis genes were associated with the diversity of secondary metabolites in F. fujikuroi strains. These findings provide insight into the population-level characterization of the FFSC and might be helpful in the development of strategies for the management of diseases and mycotoxins.