The CCR4 gene from Saccharomyces cerevisiae is required for both nonfermentative and spt-mediated gene expression.
The CCR4 gene from Saccharomyces cerevisiae is required for both nonfermentative and spt-mediated gene expression.
复制标题
来自酿酒酵母的 CCR4 基因是非发酵和 spt 介导的基因表达所必需的。
DOI：
10.1093/genetics/124.2.283
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Malvar,T
中科院分区：
文献类型：
--
作者：
Denis,CL;Malvar,T
酵母CCR 4基因突变抑制葡萄糖抑制性乙醇脱氢酶（ADH 2）以及其他非发酵基因的表达，并抑制由cre 1和cre 2等位基因引起的ADH 2表达增加。cre 1和ccr 4等位基因都通过改变ADH 2 mRNA水平来影响ADH II酶活性。CRE 1或CRE 2的突变绕过了由ADH 2启动子处的delta插入引起的ADH 2表达的抑制，所述delta插入取代了TATA元件上游336 bp的ADH 2激活序列。ccr 4等位基因可抑制cre 1和cre 2效应。当TATA元件上游的所有ADH 2调节序列被删除时，cre 1和ccr 4突变也影响ADH 2表达。研究了CRE基因与SPT基因的关系，当突变时，SPT基因能够绕过由δ启动子插入（his 4 -912 δ等位基因）引起的对HIS 4表达的抑制。cre 1和cre 2突变都允许his 4 -912 delta表达。CCR 4突变能够抑制CRE等位基因增加His 4 -912 δ表达的能力。CRE 2与SPT 6基因等位，CRE 1与SPT 10基因等位。我们认为，CRE基因包括一个一般的转录控制系统，需要在酵母中的CCR 4基因的功能。
Mutations in the yeast CCR4 gene inhibit expression of the glucose-repressible alcohol dehydrogenase (ADH2), as well as other nonfermentative genes, and suppress increased ADH2 expression caused by the cre1 and cre2 alleles. Both the cre1 and ccr4 alleles were shown to affect ADH II enzyme activity by altering the levels of ADH2 mRNA. Mutations in either CRE1 or CRE2 bypassed the inhibition of ADH2 expression caused by delta insertions at the ADH2 promoter which displace the ADH2 activation sequences 336 bp upstream of the TATA element. These cre1 and cre2 effects were suppressible by the ccr4 allele. The cre1 and ccr4 mutations also affected ADH2 expression when all the ADH2 regulatory sequences upstream of the TATA element were deleted. The relationship of the CRE genes to the SPT genes, which when mutated are capable of bypassing the inhibition of HIS4 expression caused by a delta promoter insertion (his4-912 delta allele), was examined. Both the cre1 and cre2 mutations allowed his4-912 delta expression. ccr4 mutations were able to suppress the ability of the cre alleles to increase his4-912 delta expression. CRE2 was shown to be allelic to the SPT6 gene, and CRE1 was found to be allelic to SPT10. We suggest that the CRE genes comprise a general transcriptional control system in yeast that requires the function of the CCR4 gene.