Transforming growth factor β's 1, 2 and 3 inhibit proliferation of ramified microglia on an astrocyte monolayer
Transforming growth factor β's 1, 2 and 3 inhibit proliferation of ramified microglia on an astrocyte monolayer
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DOI：
10.1016/s0006-8993(98)00325-4
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发表时间：
1998-06-08
期刊：
影响因子：
2.9
通讯作者：
Raivich, G
中科院分区：
文献类型：
--
作者：
Jones, LL;Kreutzberg, GW;Raivich, G
转化生长因子β（TGF β）是具有强免疫抑制和神经营养作用的多能细胞因子家族。在目前的研究中，我们研究了TGF β 1、2和3对在汇合的星形胶质细胞单层上培养的分枝小胶质细胞增殖的影响。所有三种TGF β亚型都抑制增殖。PCR分析还显示，在小胶质细胞、星形胶质细胞和共培养物中存在I型和II型TGF β受体以及所有3种TGF β亚型的mRNA。此外，用抗TGF β 1和TGF β 3的抗体去除这种内源性TGF β活性强烈刺激小胶质细胞增殖。这些对分支小胶质细胞增殖的抑制作用表明，TGF β在正常条件下和中枢神经系统损伤或疾病后对小胶质细胞群体的调节中可能起重要作用。(C)1998 Elsevier Science B.V.保留所有权利。
The transforming growth factor beta's (TGF beta) are a multipotent family of cytokines with strong immunosuppressive and neurotrophic effects. In the current study, we examined the effect of the TGF beta's 1, 2 and 3 on the proliferation of ramified microglia cultured on top of a confluent astrocyte monolayer. All three TGF beta isoforms inhibited proliferation. PCR analysis also showed the presence of mRNA for the TGF beta receptors type I and II and for all 3 TGF beta isoforms in microglia, astrocytes and in co-cultures. Moreover, removal of this endogenous TGF beta activity with antibodies against TGF beta 1 and TGF beta 3 strongly stimulated microglial proliferation. These inhibitory effects on the proliferation of ramified microglia suggest that TGF beta's may play an important role in the regulation of the microglial population under normal conditions and after injury or disease in the central nervous system. (C) 1998 Elsevier Science B.V. All rights reserved.