Induction of intracellular calcium oscillations in human skin fibroblast populations by sinusoidal extremely low-frequency magnetic fields (20 Hz, 8 mT) is dependent on the differentiation state of the single cell.
Induction of intracellular calcium oscillations in human skin fibroblast populations by sinusoidal extremely low-frequency magnetic fields (20 Hz, 8 mT) is dependent on the differentiation state of the single cell.
复制标题
通过正弦极低频磁场（20 Hz，8 mT）诱导人皮肤成纤维细胞群中的细胞内钙振荡取决于单细胞的分化状态。
DOI：
10.2307/3579770
复制
发表时间：
1999
影响因子：
3.4
通讯作者：
Hugo Hammerle
中科院分区：
文献类型：
--
作者：
M. Löschinger;S. Thumm;Hugo Hämmerle;H. Rodemann;Monika Loschinger;Hugo Hammerle
实验分析了短期暴露于正弦极低频电磁场(20赫兹，8mT)是否会改变二倍体人皮肤成纤维细胞内钙离子的动态变化。在异质成纤维细胞群体中，约30%的细胞在40min内出现细胞内钙振荡活性的变化。在单个细胞水平上证明成纤维细胞群体对极低频率电磁场的反应取决于暴露细胞的特定分化状态。所获得的数据清楚地表明，有丝分裂前体成纤维细胞对钙动力学的反应增强，而在有丝分裂后的纤维细胞中，观察到当细胞与次适浓度的血小板衍生生长因子共同刺激时，钙动力学降低。因此，我们实验室关于极低频电磁场诱导的末端分化的数据可能与本文报道的钙离子动力学变化有关。
Experiments were performed to analyze whether short-term exposure to a sinusoidal extremely low-frequency electromagnetic field (20 Hz, 8 mT) can alter the dynamics of intracellular calcium in diploid human skin fibroblasts. In heterogeneous fibroblast populations, about 30% of the cells responded with a change in the oscillation activity of intracellular calcium within 40 min. It was demonstrated at the level of the single cell that the responsiveness of fibroblast populations to extremely low-frequency electromagnetic fields depends on the specific differentiation state of the exposed cell. The data obtained clearly indicate that mitotic progenitor fibroblasts respond with an enhancement of the dynamics of calcium, whereas in postmitotic fibrocytes a reduction of the dynamics was observed when the cells were co-stimulated with suboptimal concentrations of platelet-derived growth factor. Thus data from our laboratory on terminal differentiation induced by extremely low-frequency electromagnetic fields may be correlated with changes in the dynamics of Ca2+ reported here.