Anatomical and functional relationships between deep cerebellar nuclei and cerebellar cortical Crus II in vivo in mice
Anatomical and functional relationships between deep cerebellar nuclei and cerebellar cortical Crus II in vivo in mice
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DOI：
10.1016/j.neulet.2015.10.064
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发表时间：
2016-01
影响因子：
2.5
通讯作者：
N. Ding;Hua Jin;Bin-bin Zhang;A. Guo;Jin-Di Shi;Jun-Yang Feng;Jia Li;Xuan-Xi Shen;Yu Shi;De-Lai Qiu;Chunping Chu
中科院分区：
文献类型：
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作者：
N. Ding;Hua Jin;Bin-bin Zhang;A. Guo;Jin-Di Shi;Jun-Yang Feng;Jia Li;Xuan-Xi Shen;Yu Shi;De-Lai Qiu;Chunping Chu
我们曾报道，吹气刺激同侧须垫可引起小脑皮质第二脚叶的分子层和浦肯野细胞层的反应。采用顺行追踪和电生理方法，研究了小鼠小脑皮质第二脚三叉神经触觉反应区与小脑深核（DCN）的解剖和功能关系。结果发现，触觉激活的前额叶皮层轴突投射到同侧小脑间质核（ICN）的前部（IntA）、后部（IntP）和背外侧丘（IntDL）。在ICN中，触觉刺激诱发场电位表达了两个负分量N1和N2的序列，而ICN神经元的细胞外记录显示，响应于触觉刺激的尖峰频率增加。当面部吹气刺激持续时间≥30 ms时，ICN出现刺激关闭反应（罗夫），但刺激持续时间的增加对Ron（N1和N2）和罗夫的幅值无明显影响。ICN的N1潜伏期和达峰时间显著短于ML的N1，而ICN的N2潜伏期和达峰时间显著晚于ML的P1。目前的研究结果表明，面部感觉信息，至少部分地，从脚II，引起兴奋的ICN神经元的PC轴突传递到ICN。
We previously reported that an air-puff stimulation on the ipsilateral whisker pad evoked responses in molecular layer (ML) and Purkinje cell (PC) layer in cerebellar cortex folium Crus II. We used anterograde tracing and electrophysiological methods to investigate the anatomical and functional relationships between the trigeminal tactile response area in the cerebellar cortex Crus II and deep cerebellar nuclei (DCN) in living mice. We found that the axons of tactile activated PCs projected in anterior part (IntA) and posterior part (IntP), and dorsolateral hump (IntDL) of ipsilateral interposed cerebellar nucleus (ICN). In ICN, the tactile stimulus evoked-field potential expressed a sequence of two negative components N1 and N2, while extracellular recordings from ICN neurons revealed that an increase in spike frequency in response to tactile stimulus. When the duration of facial air-puff stimulus were ≥30 ms, stimulation off response (Roff) were observed in the ICN, but an increase in the duration of facial air-puff stimulation did not significantly affect the amplitude ofRon(N1 and N2) andRoff. The latency and time to peak of N1 in ICN were significantly shorter than that of N1 in the ML, but the latency and time to peak of N2 in ICN were significantly later than that of P1 in the ML. The present results suggest that the facial sensory information, at least in part, is transferred to ICN by PC axons from Crus II, which evokes excitation in ICN neurons.