ESTABLISHMENT AND CHARACTERIZATION OF A HUMAN PRIMITIVE NEUROECTODERMAL TUMOR-CELL LINE FROM THE CEREBRAL HEMISPHERE
ESTABLISHMENT AND CHARACTERIZATION OF A HUMAN PRIMITIVE NEUROECTODERMAL TUMOR-CELL LINE FROM THE CEREBRAL HEMISPHERE
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DOI：
10.1097/00005072-199205000-00005
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发表时间：
1992-05-01
影响因子：
3.2
通讯作者：
MCKAY, RDG
中科院分区：
文献类型：
--
作者：
FULTS, D;PEDONE, CA;MCKAY, RDG
原始神经外胚层肿瘤（PNET）包括一类折磨儿童的恶性神经系统肿瘤。这些肿瘤由与神经胚胎发育早期通常可见的原始神经上皮细胞形态相同的细胞组成，支持PNET是由正常神经元或神经胶质分化过程中的干扰引起的观点。在中枢神经系统中，PNET最常发生在小脑（髓母细胞瘤），但偶尔发生在大脑半球。我们在这里报告的建立和表征的一个新的人类细胞系（PFSK）来自PNET从一个孩子的大脑半球。PFSK细胞的生长特征是典型的永生化转化细胞系。细胞遗传学和分子遗传学研究表明，存在三个不同的亚系。在其中一个亚系中，17号染色体的序列在培养过程中丢失。免疫细胞化学研究表明，PFSK细胞表达巢蛋白，神经上皮干细胞在神经形成过程中通常表达的中间丝蛋白。PFSK细胞不表达终末分化的神经元或神经胶质中常见的抗原，表明该肿瘤细胞系可能代表神经上皮干细胞，然后再分化为神经元或神经胶质细胞系。
The primitive neuroectodermal tumors (PNET) comprise a class of malignant nervous system neoplasms that afflict children. These tumors consist of cells that are morphologically identical to the primitive neuroepithelial cells normally seen in early stages of neural embryogenesis, supporting the notion that PNET result from a disturbance in the process of normal neuronal or glial differentiation. In the central nervous system, PNET occur most commonly in the cerebellum (medulloblastomas), but only occasionally in the cerebral hemispheres. We report here the establishment and characterization of a new human cell line (PFSK) derived from a PNET from the cerebral hemisphere of a child. The growth characteristics of PFSK cells were typical of an immortalized, transformed cell line. Cytogenetic and molecular genetic studies showed that three different sublines were present. In one of these sublines, sequences from chromosome 17 had been lost during establishment in culture. Immunocytochemical studies showed that PFSK cells expressed nestin, an intermediate filament protein normally expressed by neuroepithelial stem cells during neurulation. The PFSK cells did not express antigens typically found in terminally differentiated neurons or glia, indicating that this tumor cell line might represent neuroepithelial stem cells prior to commitment to a neuronal or glial lineage.