13-Hydroxyoctadecadienoic acid is the vessel wall chemorepellant factor, LOX.
13-Hydroxyoctadecadienoic acid is the vessel wall chemorepellant factor, LOX.
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13-羟基十八碳二烯酸是血管壁化学排斥因子，LOX。
DOI：
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发表时间：
1985
影响因子：
4.8
通讯作者：
M. Guichardant
中科院分区：
文献类型：
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作者：
Michael R. Buchanan;Haas Ta;Michel Lagarde;M. Guichardant
我们以前曾报道，内皮细胞合成的细胞溶质相关，脂氧合酶衍生的代谢产物，LOX，它作为一种化学排斥剂，并在这样做，保持血管壁血栓阻力。在这项研究中，我们证明，LOX是一种13-羟基亚油酸（13-OH-18：2），来自亚油酸和相同的13-羟基-9-顺，11-反式-十八碳二烯酸，通过反相高压液相色谱法和气相色谱/质谱法测定。此外，我们证明，13-OH-18：2是由内皮细胞比平滑肌细胞或成纤维细胞产生的显着更大的数量。此外，我们表明，13-OH-18：2是在基础条件下产生的微克量，并减少凝血酶，钙离子载体，胰蛋白酶刺激。最后，我们证明，内皮细胞不合成任何显着量的脂氧合酶衍生的花生四烯酸代谢产物，无论是在基础或刺激条件下，除非外源性花生四烯酸被添加。这些观察结果表明，由内皮细胞产生的主要脂氧合酶衍生的化学排斥代谢物是13-羟基-9-顺式，11-反式-十八碳二烯酸。
We have previously reported that endothelial cells synthesize a cytosol-associated, lipoxygenase-derived metabolite, LOX, which acts as a chemorepellant and, in so doing, maintains the vessel wall thromboresistance. In this study we demonstrate that LOX is a 13-hydroxylinoleic acid (13-OH-18:2) derived from linoleic acid and identical to 13-hydroxy-9-cis,11-trans-octadecadienoic acid, as measured by both reverse phase high pressure liquid chromatography and gas chromatography/mass spectrometry. In addition, we demonstrate that 13-OH-18:2 is produced in significantly greater quantities by endothelial cells than by smooth muscle cels or by fibroblasts. Furthermore, we demonstrate that 13-OH-18:2 is produced in microgram amounts under basal conditions and is decreased by thrombin, calcium ionophore, and trypsin stimulation. And finally, we demonstrate that endothelial cells do not synthesize any significant amounts of lipoxygenase-derived arachidonic acid metabolites either under basal or stimulated conditions unless exogenous arachidonic acid is added. These observations indicate that the major lipoxygenase-derived, chemorepellant metabolite produced by the endothelial is 13-hydroxy-9-cis,11-trans-octadecadienoic acid.